Abstract: High-throughput drug screening was applied to study the butyrylcholinesterase (BuChE) inhibitory activity of Chinese herb extracts and to analyze the active ingredients were carried out.The constituents in Paederia scandens (lour.) Merr.were determined by UPLC-Q-TOF-MS.Herbs were percolated with petroleum ether,ethanol extract,ethyl acetate extraction and boiling water,to obtain the four corresponding parts of extraction.BuChE inhibitory activity of the candidate compounds was assayed based on thionitrobenzoicanion (DTNB) method.The chromatographic separation was performed on a C₁₈ column (2.1 mm×50 mm,1.7 μm) with a gradient elution of methanol-water.The mass spectrometer equipped with electron spray ionization source was used as detector and the data was collected under the positive ion modes.57 Chinese herb extracts showed BuChE inhibitory activity,with the ethyl acetate extraction part of Paederia scandens (lour.) Merr.showing a strong activity.Nine constituents were analyzed and identified,including paederoside，paederosidic acid，methylpaederosidate，caffeic acid 4-O-β-D-glucopyranoside paederoside B as the main active components,and linarin as a flavonoid composition,and 4 unknown substances.Parts of Paederia scandens (lour.) Merr.extracted with ethyl acetate has significant BuChE inhibitory activity,and IC₅₀ was 12.415 μg/mL.Iridoids were identified through the information of positive ion and relative molecular mass which were determined by Q-TOF-MS.The results indicate that Paederia scandens (lour.) Merr.contains iridoids ingredients as inhibitors of BuChE,which lays the foundation for further tracking separation of active ingredients.