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白杨素对人鼻咽癌细胞CNE-2增殖的抑制作用

Effect of chrysin in inhibiting proliferation of human nasopharyngeal carcinoma cell line CNE-2

  • 摘要: 探讨白杨素对人鼻咽癌细胞CNE-2增殖的抑制作用及机制。采用CCK-8法检测细胞存活率,Hochest 33258染色检测细胞形态变化,流式细胞仪检测细胞凋亡率及细胞周期的变化,caspase-3活性检测试剂盒检测caspase-3的活性。结果表明白杨素对CNE-2细胞的生长具有较强的抑制作用,并具有明显的量-效关系;白杨素可诱导CNE-2细胞凋亡,使G0/G1期细胞明显增多,S期明显减少,使细胞周期被阻滞于G0/G1期;白杨素可升高CNE-2细胞caspase-3的活性。白杨素对CNE-2细胞生长有明显的抑制作用,其机制可能与白杨素诱导的细胞周期阻滞和细胞凋亡有关。

     

    Abstract: The aim of the study was to investigate the inhibition effect of chrysin on the growth of human nasopharyngeal darcinoma CNE-2 cells.The cell proliferation was determined by CCK-8;the apoptosis bodies were observed by Hoechst 33258 staining;the apoptosis was detected by flow cytometry;and the activity of caspase-3 was analyzed by caspase-3 activity assay kit.The proliferation of CNE-2 cells was inhibited by chrysin in the concentration and time-dependent manners.Chrysin induced the apoptosis of CNE-2 cells.Furthermore,chrysin dramatically induced G0/G1 phase cell increase and S phase cell decrease.Cell cycle was arrested at G0/G1 phase.Chrysin could elevate the caspase-3 activity observably.Chrysin markedly inhibited the proliferation of CNE-2 cells.The inhibition effect may be related to inducing the apoptosis and cell cycle arrest on CNE-2 cells.

     

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