HZ08冻干脂质体的制备及其逆转肿瘤多药耐药的研究

陈京; 饶光玲; 黄静; 葛亮; 施秦

doi:10.11665/j.issn.1000-5048.20130206

HZ08冻干脂质体的制备及其逆转肿瘤多药耐药的研究

Preparation of lyophilized HZ08 liposomes and the reversal of multidrug resistance in tumor

摘要

摘要: 制备了HZ08冻干脂质体,考察脂质体冻干前后的包封率、粒径和长期放置稳定性等指标。通过K562/A02细胞逆转阿霉素耐药性实验,考察了HZ08冻干脂质体对逆转阿霉素耐药性作用。结果表明：制备的HZ08脂质体冻干前后包封率、粒径等指标基本相同。12周的放置实验，HZ08冻干脂质体的药物渗漏率为未冻干的25.92%，且脂质体粒径的增幅仅为未冻干的12.32%，冻干后的脂质体稳定性显著提高。10，3，1 μmol/L HZ08脂质体冻干粉对K562/A02细胞具有不同程度的逆转阿霉素的耐药性作用，其逆转倍数分别为50.87、17.75和2.05。分别于3个月、6个月和12月时，取样测定批量生产的HZ08冻干脂质体的各项指标，各项指标无明显变化。

Abstract: HZ08 liposomes (HL) and lyophilized HZ08 liposomes (LHL) were prepared and characterized by encapsulation efficiency (EE),particle size (PS) and long term stability.The effect of LHL on reversal of adriamycin resistance was investigated by K562/A02 cell reversal of adriamycin resistance experiment.It was found that the PS and EE had no significant change after freeze-drying of HL.However,leakage rate of LHL (12.32%) was significantly lower than that of LHL (25.92%) after 12-weeks storage.The effect of LHL on the reversal of adriamycin resistance was investigated by K562/A02 cell reversal with different concentration LHL(10,3,1 μmol/L),and the reversal fold was 50.87,17.75 and 2.05,respectively.In 3 months,6 months and 12 months,LHL was sampled to determine the quality,and there was no obvious change during the storage.

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