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甲哌啶在大鼠体内的分布研究

In vivo postmortem distribution of mepiquat chloride in rats

  • 摘要: 建立生物检材中甲哌啶的超高效液相色谱 质谱联用检测方法,研究甲哌啶(mepiquat chloride,MQ)灌胃染毒致死的大鼠动物模型。大鼠以1/2LD50剂量(200 mg/kg原药浓度)甲哌啶灌胃染毒,分别于染毒后3,8,12 h处死解剖,采集心、肝、脾、肺、肾、脑、膀胱、肌肉、小肠和胃组织,UPLC-MS/MS法定量检测各组织中甲哌啶浓度。试验中大鼠灌胃后,12 h内小肠、胃、膀胱、肾是主要分布器官。肺中含量较低,小肠含量最多。8 h内各脏器中含量变化不大,8 h后组织内甲哌啶含量急剧下降。各组织与脑组织比较具有显著性差异(P<0.05)。结果显示甲哌啶在大鼠体内死后分布不均匀并且各组织含量随着时间变化有所改变。甲哌啶口服染毒的致死动物模型、UPLC-MS/MS方法以及各组织分布规律可作为甲哌啶中毒死亡案件的法医学鉴定参考依据,并且为全面正确采取检材进行毒物分析提供方向。

     

    Abstract: A UPLC-MS/MS method was established for qualitative detection and quantitative determination of mepiquat chloride in the bio-samples.A poisoned dead rat model was established by intragastric administration of 1/2LD50 mepiquat chloride(200 mg/kg).Then the rats would be dissected at 3rd ,8th ,and 12th hour to collect the rats′ specimen(heart,liver,spleen,lung,kidney,brain,muscle,small intestine,stomach),and analyzed by UPLC-MS/MS immediately.After ig administration in the rats,small intestine ,stomach,bladder,kidney were the main distribution organs within 12 hours with low levels in the lungs and the highest level in the small intestine.The concentration of these visceras changed little within 8 hours,but had a sharp decline after 8 hours .Each viscera had significant difference in contrast to the brain(P<0.05).It was found that there was a postmortem maldistribution of mepiquat chloride in these rat models and the concentration of visceras changed over time.The poisoned dead rat model,the UPLC-MS/MS method and the postmortem distribution of mepiquat chloride can be applied to the forensic identification and help taking samples for toxicology analysis.

     

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