不同表面性质纳米脂质体对肝肿瘤细胞摄取的影响
Influence of surface characteristics on hepatocellular carcinoma cells uptake of nano-liposomes
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摘要: 以紫杉醇为模型药物，选用不同的脂质材料制备不同电性和粒径的紫杉醇脂质体，考察其体外药物释放特性。通过细胞摄取实验研究不同表面性质脂质体在肝肿瘤细胞系中的摄取。制剂的体外释药实验显示，48 h累积释放量均不超过30%，具有缓释特征。采用MTT法考察了制剂的细胞毒性，发现紫杉醇质量浓度低于50 μg/mL时，细胞48 h存活率不低于80%。比较了两种肿瘤细胞摄取能力的差异，并测定了肝肿瘤细胞BEL-7402细胞和HepG2细胞48 h内对药物的摄取。结果表明，随着制剂粒径的减小和表面Zeta电位的增大，细胞摄取药物增加。Abstract: Liposomes with different surface characteristics were prepared based on different lipid materials and preparation conditions. Paclitaxel(PTX)was selected as the model drug. The release characteristics of these liposomes were investigated. The uptaken of liposomes of different surface properties in hepatocellular carcinoma cells were studied through cellular uptaken experiment. All these liposomes showed sustained release property, as their accumulative release amounts were less than 30% in 48 h. The cytotoxicity of every preparation was evaluated with MTT method. Hepatocellular carcinoma cells survived more than 80% when the concentration of PTX was below 50 μg/mL in 48 h. The effect of different surface characteristics on uptake of liposomes by hepatocellular carcinoma cell lines(BEL-7402 cells and HepG2 cells)was investigated through quantification of PTX in cells. Cell uptake results indicated that preparations with smaller size or higher Zeta potential showed more PTX uptake.
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Keywords:
- paclitaxel /
- liposomes /
- surface characteristics /
- release /
- hepatocellular carcinoma cell /
- cell uptake
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