Abstract: Liposomes with different surface characteristics were prepared based on different lipid materials and preparation conditions. Paclitaxel(PTX)was selected as the model drug. The release characteristics of these liposomes were investigated. The uptaken of liposomes of different surface properties in hepatocellular carcinoma cells were studied through cellular uptaken experiment. All these liposomes showed sustained release property, as their accumulative release amounts were less than 30% in 48 h. The cytotoxicity of every preparation was evaluated with MTT method. Hepatocellular carcinoma cells survived more than 80% when the concentration of PTX was below 50 μg/mL in 48 h. The effect of different surface characteristics on uptake of liposomes by hepatocellular carcinoma cell lines(BEL-7402 cells and HepG2 cells)was investigated through quantification of PTX in cells. Cell uptake results indicated that preparations with smaller size or higher Zeta potential showed more PTX uptake.