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超高效液相色谱法同时测定藿香正气水中7种成分含量

Simultaneous determination of seven components in Huoxiang Zhengqi liquid by ultra-performance liquid chromatography

  • 摘要: 利用超高效液相色谱同时测定藿香正气水中7种有效成分的含量。采用Acquity BEH C18(2.1 mm×100 mm,1.7 μm)色谱柱,以乙腈-0.1%磷酸溶液线性梯度洗脱,柱温35 ℃,流速0.4 mL/min,分别在249,284,336 nm测定7种有效成分含量,12 min内完成测试。结果显示,甘草酸、欧前胡素、异欧前胡素、橙皮苷、厚朴酚、和厚朴酚、苍术素等7种成分在各自线性范围呈良好的线性关系(r>;0.999 9),加样回收率在96.16%~103.4%之间。

     

    Abstract: An ultra-performance liquid chromatographic method was established for the simultaneous determination of seven active compounds in Huoxiang Zhengqi liquid. Seven compounds were analyzed simultaneously with an Acquity BEH C18 column by gradient elution using acetonitrile-0. 1% phosphoric acid as the mobile phase. The column was maintained at 35 °C; the flow rate was 0. 4 mL/min; the detection wavelengths were set at 249, 284 and 336 nm, respectively. Good linearity(r> 0. 999 9)in the range of the test concentration was observed between concentration and chromatographic response of each compound, and the average recoveries of the method were between 96. 16% and 103. 4%.

     

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