超高效液相色谱法同时测定藿香正气水中7种成分含量

基金项目: 河北省自然科学基金-石药集团医药联合研究基金资助项目(No.C2011320007)

Simultaneous determination of seven components in Huoxiang Zhengqi liquid by ultra-performance liquid chromatography

摘要: 利用超高效液相色谱同时测定藿香正气水中7种有效成分的含量。采用Acquity BEH C₁₈(2.1 mm×100 mm，1.7 μm)色谱柱，以乙腈-0.1%磷酸溶液线性梯度洗脱，柱温35 ℃，流速0.4 mL/min，分别在249，284，336 nm测定7种有效成分含量，12 min内完成测试。结果显示，甘草酸、欧前胡素、异欧前胡素、橙皮苷、厚朴酚、和厚朴酚、苍术素等7种成分在各自线性范围呈良好的线性关系(r>；0.999 9)，加样回收率在96.16%~103.4%之间。
- 超高效液相色谱 /
- 藿香正气水 /
- 含量测定
Abstract: An ultra-performance liquid chromatographic method was established for the simultaneous determination of seven active compounds in Huoxiang Zhengqi liquid. Seven compounds were analyzed simultaneously with an Acquity BEH C₁₈ column by gradient elution using acetonitrile-0. 1% phosphoric acid as the mobile phase. The column was maintained at 35 °C; the flow rate was 0. 4 mL/min; the detection wavelengths were set at 249, 284 and 336 nm, respectively. Good linearity(r> 0. 999 9)in the range of the test concentration was observed between concentration and chromatographic response of each compound, and the average recoveries of the method were between 96. 16% and 103. 4%.
- ultra performance liquid chromatography /
- Huoxiang Zhengqi liquid /
- determination

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