Abstract: The aim of study was to determine the relationship between the biomarker of glucose and lipid metabolism and N^G, N^G′-asymmetric dimethylarginine(ADMA), total homocysteine(tHcy), adiponectin(APN)and pentraxin 3(PTX₃)in type 2 diabetes mellitus rats. Diabetes was induced in Sprague-Dawley rats by a single intraperitoneal injection of 35mg/kg streptozotocin(STZ). Before and 2nd, 4th, 7th, 9th weeks after STZ injection, fasting plasma glucose(FPG), fasting insulin(FINS), OGTT, serum lipid(TC, TG and LDL-C), ADMA, tHcy, APN, PTX₃ were determined. Compared with the groups of control and high fat diet(HFD), FPG, Homeostasis model assessment for insulin resistence index(HOMA-IR), TC, TG and LDL-C level of type 2 diabetes mellitus(T2DM)groups were significantly increased. In T2DM group, the concentration of ADMA increased gradually, tHcy decreased by degrees, APN reduced significantly, the PTX₃ elevated remarkably. Our results indicate that ADMA, tHcy, APN, PTX₃ are well correlated with glucose and lipid metabolism biomarkers. The plurality of biomarkers mentioned before should be monitored to evaluate the cardiovascular risk of type 2 diabetes mellitus.