姜黄油诱导人肝癌细胞凋亡及其机制

Turmeric oil induces human hepatoma cell apoptosis via mitochondrial pathway

摘要: 探讨姜黄油的抗肝癌作用及其作用机制，采用二甲基噻唑蓝比色法(MTT)检测肝癌细胞的活力，流式细胞仪检测细胞凋亡率以及线粒体膜电位的变化，Western blot方法检测凋亡相关蛋白Bax、Bcl-2、caspase-3、caspase-9以及细胞色素C的表达。结果发现，姜黄油能时间依赖性地抑制肝癌细胞的生长，降低线粒体膜电位并诱导其凋亡；升高细胞内Bax与Bcl-2的比值；诱导细胞色素C从线粒体中的释放并激活caspase-9和caspase-3。结果提示姜黄油可能通过线粒体途径诱导肝癌细胞的凋亡。
- 姜黄油 /
- 肝癌 /
- 凋亡
Abstract: To evaluate the anti-human hepatocellular carcinoma activity of turmeric oil(TO). Cell viability was assessed by MTT assay, cell apoptosis and mitochondrial membrane potential were measured by flow cytometry. The protein abundance of Bax, Bcl-2, caspase-3, caspase-9 and cytochrome C was analyzed by Western blot. The results demonstrated TO-induced apoptosis of Bel-7402 cells in a time- and dose- dependent manners. It was characterized by loss of mitochondrial membrane potential(ΔΨ_m)and enhanced Bax/Bcl-2 ratio. The release of cytochrome C from mitochondria and the activation of caspase-9 and -3 indicated that TO might induce apoptosis of Bel-7402 cells via mitochondrial-mediated pathway.
- turmeric oil /
- hepatocellular carcinoma /
- apoptosis

1.	翟红伟，赵玉婷，林露，龙林，万丽娟，胡辉，黄茜茜. 指纹图谱和多成分定量结合化学模式识别法评价清肺达原颗粒质量. 亚太传统医药. 2024(08): 43-48 . 百度学术
2.	于洋，辛杨，王海军，杨清竹，王伟明. 基于超高效液相色谱/四极杆-静电场轨道阱质谱联合网络药理学筛选“芩百清肺浓缩丸”治疗肺炎的活性成分. 质谱学报. 2024(06): 884-896 . 百度学术
3.	王优，宋琴，穆卡然·艾买江，李佳川. 基于多元数据融合分析的彝药两头毛治疗胆汁淤积型肝损伤药效及机制研究. 西南民族大学学报(自然科学版). 2022(01): 42-52 . 百度学术
4.	Chang-Liang Yao，Wen-Long Wei，Jian-Qing Zhang，Qi-Rui Bi，Jia-Yuan Li，Ikhlas Khan，Rudolf Bauer，De-An Guo. Traditional Chinese Medicines Against COVID-19: A Global Overview. World Journal of Traditional Chinese Medicine. 2022(03): 279-313 . 必应学术
5.	胡盼盼. 基于网络药理学和分子对接研究绿原酸抗PRRSV的作用机制. 黑龙江畜牧兽医. 2021(19): 79-83+151-152 . 百度学术