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牛蒡多糖的降血糖活性

Hypoglycemic activity of a polysaccharide from the roots of Arctium lappa L.

  • 摘要: 从牛蒡根中提取纯化获得牛蒡多糖ALP1,通过体外α-葡萄糖苷酶抑制试验和体内糖尿病模型小鼠试验研究其降血糖活性。体外实验表明,ALP1具有良好的α-葡萄糖苷酶抑制活性,IC50为0.518 4 mg/mL;体内实验发现ALP1能显著降低链脲霉素诱导的糖尿病模型小鼠的血糖水平,明显改善糖耐量及胰岛组织的病变状况,降低一氧化氮合成酶的酶活力、氧自由基和丙二醛含量,提高超氧化物歧化酶的酶活力。ALP1对糖尿病小鼠显示出较好的降血糖效果,其降血糖机制可能与抑制α-葡萄糖苷酶的活性,延缓糖类在肠道内的吸收,及通过体内抗氧化作用,减少糖尿病鼠体内氧化损伤有关。

     

    Abstract: A polysaccharide coded as ALP1 was prepared and purified from Arctium lappa L. (A. lappa L. ) roots. The hypoglycemic activity of ALP1 was determined both in vitro and in vivo. In vitro hypoglycemic assays, ALP1 significantly inhibited the activity of α-glucosidase, and the IC50 of ALP1 was determined to be 0. 518 4 mg/mL. In vivo hypoglycemic assays, ALP1 significantly reduced fasting blood glucose level, improved glucose tolerance and islet tissue lesions, reduced nitric oxide synthetase activity, decreased reactive oxygen species and malondialdehyde levels, whereas enhancing the activity of superoxide dismutase. The study indicated that ALP1 has good hypoglycemic effect on diabetic mice, and the mechanism may be related to slowing down the absorption of carbohydrates in the intestine by inhibiting the activity of α-glucosidase and reducing the oxidative damage of diabetic mice.

     

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