Abstract: This study investigated the influence of snogE disruption in biosynthesis pathway of nogalamycin. According to the sequence alignment of several glycosytransferases, we cloned the fragment of snogE gene sharing the highest homology with the published anthracycline antibiotics glycosyltransferase sequence. The gene disruption plasmid pSXW-2-62 was constructed and transferred into Streptomyces nogalater ATCC27451 by conjugation. Finally, we got the recombinant strain mSXW-2-71 by homologous recombination. The genotype and fermentation product of mSXW-2-71 were identified. As a result, the gene disruption plasmid was integrated into the genome and interrupted snogE gene successfully. Nogalymycin could not be detected in fermentation broth. The snogE encoding glycosyltransferase is necessary in nogalamycin biosynthesis pathway.