Abstract: Andrographolide(Andro)is one of the major components extracted from Andrographis paniculata. As a natural plant medicine, Andro has been used to treat inflammation for thousands of years in Asia and South America. It has been reported to exhibit an important effect on tumor growth, yet its inhibition of Tb cell apoptosis has never been reported. To obtain the effect of Andro on Tb cell apoptosis, Swiss Giemsa staining, Hoechst 33258 dying, in situ Annexin V/PI double staining and FCM were used. Furthermore, its mechanisms of action on Tb cell apoptosis by Western blot were detected. It was found that Tb cell apoptosis rate and PARP expression level significantly increased after Andro treatment. Moreover, Andro promoted the Tb cell apoptosis through activating Caspase-3/PARP.