单纯性浅表皮肤感染小鼠模型的建立及有效性验证
Establishment of a simple superficial skin infection model in mice and verification of its effectiveness
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摘要: 建立与临床上单纯性浅表皮肤感染较为接近的小鼠模型，便于致病机制和外用抗菌药物筛选及疗效观察研究。选用BALB/c小鼠，采用胶带反复粘贴法破坏局部皮肤屏障，通过皮肤水分流失(TEWL)值标定皮肤屏障破坏程度，然后在其创面接种金黄色葡萄球菌(7.5×106 CFU/mL)及化脓性链球菌(1.5×108 CFU/mL)，建立局部皮肤的实验感染模型，并给予氧氟沙星凝胶以验证模型的敏感性和有效性。结果显示:胶带反复粘贴7次和10次分别为金黄色葡萄球菌和化脓性链球菌感染前皮肤屏障破坏的最佳次数；6株金黄色葡萄球菌和3株化脓性链球菌局部感染后，创面活菌培养结果分别为4.99±4.74、5.52±5.45、4.92±4.56、5.24±4.84、5.20±5.01、4.94±4.69和4.82±4.87、5.34±5.45、4.63±4.41 CFU/g，与对照组比较差异均有统计学意义(P<；0.01)。给予氧氟沙星凝胶后，对感染灶细菌生长的抑制率均大于80%。结果表明，所建立的皮肤浅表感染模型具有一定的敏感性和有效性，对皮肤浅表感染发病机制研究、外用抗菌药物筛选和疗效观察具有一定的应用前景。Abstract: The aim of this study is to establish a mouse model close to clinical simple superficial skin infection for the study of screening and therapeutic effect of topical antimicrobial agents as well as the pathogenic mechanisms. The skin barrier of BALB/c mice was disrupted with partial removal of the epidermal layer by tape stripping the topical skin in succession and TEWL values were measured to standardize the degree of barrier disruption. The topical skin was infected by Staphylococcus aureus pathogens(7. 5×106 CFU/mL)or Streptococcus pyogenes(1. 5×108 CFU/mL)after disruption of skin barrier. The sensitivity and effectiveness of superficial skin infection model was verified by treatment of skin lesions with ofloxacin gel. The results showed that tape- stripping 7 and 10 times respectively were optimal methods of destruction of skin barrier for superficial skin infection of Staphylococcus aureus and Streptococcus pyogenes. The CFU numbers recovered from the skin wound after application of 6 strains of Staphylococcus aureus and 3 strains of Streptococcus pyogenes were 4. 99±4. 74, 5. 52±5. 45, 4. 92±4. 56, 5. 24±4. 84, 5. 20±5. 01, 4. 94±4. 69, and 4. 82±4. 87, 5. 34±5. 45, 4. 63±4. 41 CFU/g, respectively. There is significant difference between the control groups and the model groups(P< 0. 01). The inhibition rates of bacterial growth in skin lesion after ofloxacin gel treatments were more than 80%. The results suggest that the established superficial skin infection model is sensitive and effective. It should be applicable to study the pathogenesis and evaluation of novel topical antimicrobial treatments of superficial skin infections caused by Staphylococcus aureus and Streptococcus pyogenes.
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Keywords:
- animal model /
- skin infection /
- tape stripping /
- Staphylococcus aureus /
- Streptococcus pyogenes
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