GC法测定痛宁凝胶中冰片和薄荷脑的含量
Determination of borneol and menthol in Tongning gel by GC
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摘要: 采用GC法同时测定痛宁凝胶中龙脑、异龙脑和薄荷脑的含量。采用5%二苯基-95%二甲基硅烷共聚物为固定相的毛细管色谱柱(30 m×0.32 mm×0.2 μm)，分流比为50∶1，柱温为88 ℃。结果表明痛宁凝胶中的龙脑、异龙脑和薄荷脑的分离度和线性关系良好，线性范围依次为0.116 9~1.402 8 mg/mL、0.107 8~1.293 6 mg/mL和0.112 7~1.352 4 mg/mL；加样回收率依为98.02%(RSD=1.76%)、100.00%(RSD=1.78%)和101.77%(RSD=1.33%)。所建立的方法适合于痛宁凝胶的质量控制。Abstract: To establish a GC method for the determination of borneol, isobornel and mentol in Tongning gel. The GC column was packed with 5% diphenyl and 95% dimenthyl polysiloxanc(30 m×0. 32 mm×0. 2 μm), and the split ratio and column temperature were 50 ∶1 and 88 °C, respectively. The results showed that borneol, isoborneol and menthol in Tongning gel were well-separated. The calibration curves were linear over the concentration ranger of 0. 116 9-1. 402 8mg/mL, 0. 107 8-1. 293 6mg/mL and 0. 112 7-1. 352 4 mg/mL for borneol, isoborneol and menthol, respectively. The average recoveries were 98. 02%(RSD=1. 76%), 100. 00%(RSD=1. 78%)and 101. 77%(RSD=1. 33%)respectively. The established method is accurate, sensitive and reproducible in the quality control of Tongning gel.
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Keywords:
- GC /
- menthol /
- borneol /
- isoborneol /
- Tongning gel /
- determination
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[1] Chinese Pharmacopoeia Commission.Chinese Pharmacopoeia:part 1.[M].Beijing:Chinese Medicine Science Press(中国医药科技出版社),2010:136-137,395-396. [2] Fu XH.Capillary gas chromatography simultaneous determination of menthol,methyl salicylate and synthetic borneol in Shaolin Fengshi Dieda extract[J].Chin J Pharm Anal(药物分析杂志),2011,31(6):1 111-1 115. [3] Liu X Sun F.Determination of camphor,menthol,synthetic borneol and wintergreen in Shexiang Zhuanggu plaster by GC[J].Chin Tradit Pat Med(中成药),2009,31(2):231-234. [4] Sun XM,Dai DM,Cheng XL,et al.Simultaneous determination of camphor,menthol synthetic borneol and methyl salicylate in Shexiang Zhuanggu plaster by GC method[J].Chin Tradit Pat Med(中成药),2007,29(7):1 004-1 008. [5] Zhou Z,Wei JJ.GC determination of mentholum camphora borneolum syntheticum menthyl salicylate and thymol in Wuji cream[J].Chin J Pharm Anal(药物分析杂志),2007,7(7):1 072-1 074. [6] Li MY.Determination of borneol content in Niuhuang Jiedu tablets by capillary gas chromatography[J].China Pharm(中国药业),2009,18(5):25-26.
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