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自组装法制备茶多酚-明胶-壳聚糖纳米粒及其性质表征

张茵, 孟晨, 常俊, 沙莎, 陈昊, 卢梭

张茵, 孟晨, 常俊, 沙莎, 陈昊, 卢梭. 自组装法制备茶多酚-明胶-壳聚糖纳米粒及其性质表征[J]. 中国药科大学学报, 2014, 45(2): 178-184. DOI: 10.11665/j.issn.1000-5048.20140209
引用本文: 张茵, 孟晨, 常俊, 沙莎, 陈昊, 卢梭. 自组装法制备茶多酚-明胶-壳聚糖纳米粒及其性质表征[J]. 中国药科大学学报, 2014, 45(2): 178-184. DOI: 10.11665/j.issn.1000-5048.20140209
ZHANG Yin, MENG Chen, CHANG Jun, SHA Sha, CHEN Hao, LU Suo. Preparation and characterization of a self-assembled tea polyphenol-gelatin-chitosan nanoparticles[J]. Journal of China Pharmaceutical University, 2014, 45(2): 178-184. DOI: 10.11665/j.issn.1000-5048.20140209
Citation: ZHANG Yin, MENG Chen, CHANG Jun, SHA Sha, CHEN Hao, LU Suo. Preparation and characterization of a self-assembled tea polyphenol-gelatin-chitosan nanoparticles[J]. Journal of China Pharmaceutical University, 2014, 45(2): 178-184. DOI: 10.11665/j.issn.1000-5048.20140209

自组装法制备茶多酚-明胶-壳聚糖纳米粒及其性质表征

基金项目: 国家自然科学基金资助项目(No.81172007);江苏省高校自然科学基金资助项目(No.09KJB180002)

Preparation and characterization of a self-assembled tea polyphenol-gelatin-chitosan nanoparticles

  • 摘要: 将茶多酚(tea polyphenol,TP)、明胶(gelatin,Gel)和壳聚糖(chitosan,Cs)自组装成茶多酚纳米粒,其中,明胶与壳聚糖通过静电结合形成可溶性复合物,茶多酚与糖基化修饰的明胶通过非共价键组装,最后形成茶多酚-明胶-壳聚糖(TP-Gel-Cs)纳米粒。经工艺筛选,3种成分的最佳质量比为1.5∶3∶1。对TP-Gel-Cs纳米粒进行理化性质、稳定性和体外释放特性评价。结果表明,TP-Gel-Cs纳米粒内存在非共价键,该体系能够有效包载茶多酚,载药量为26.82%,包封率为90.42%,平均粒径为971.50 nm,分散指数为0.22,在中性条件下荷负电,在pH 5.4条件下荷正电;在室温下,茶多酚的稳定性显著提高;TP-Gel-Cs纳米粒呈现pH敏感且缓慢的体外释放特性。该制备工艺绿色、温和、简便,可以发展为多酚类物质的纳米载药体系。
    Abstract: A kind of self-assembled tea polyphenol(TP), gelatin(Gel)and chitosan(Cs)nanoparticles(TP-Gel-Cs)was prepared and evaluated in vitro. TP-Gel-Cs nanoparticles were fabricated based on an electrostatic bonding between Gel and Cs, together with a non-covalent interaction between TP and polysaccharide modified Gel. The optimal formulation of nanoparticles was a mass ratio of 1. 5 ∶3 ∶1 for TP-Gel-Cs nanoparticles. The present experiments showed that the primary interaction exiting within the TP-Gel-Cs matrix was non-covalent bond. The mean diameter of the particles was 971. 50 nm with a polydispersity index of 0. 22, the Zeta-potential was -10. 44 mV at the neutral condition and +33. 23 mV at pH 5. 4. The entrapment efficiency and loading capacity of the particles were 90. 42% and 26. 82%, respectively. The stability of TP at 25 ℃ was significantly improved by formation of TP-Gel-Cs nanoparticles, the nanoparticles exhibit both pH-sensitive and controlled release properties. Taken together, the preparation process is simple, mild and green, which may be a promising method to encapsulation polyphenol substances.
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出版历程
  • 刊出日期:  2014-04-24

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