Abstract: An HPLC-DAD fingerprint method was developed for the simultaneous determination of Danggui-Shaoyao-San. A Welchrom C₁₈ column(250 mm×4. 6 mm, 5 μm)was used with a gradient mobile phase system of acetonitrile-water containing 0. 05% phosphoric acid. The detection wavelength was 230 nm; the flow rate was 1 mL/min. From the fingerprint we can conclude that in the total 21 peaks, 8 was from Angelicae sinensis, 11 from Paeonia lactiflora, 13 from the Ligustici Chuanxiong, 4 from Atractylodis macrocephalae, 2 from Poria cocos, and 3 from Rhizoma Alismatis. The validated method was applied to the quality control of Danggui-Shaoyao-San, providing a basis for the efficacy of various parts to clarify the material basis.