HPLC法同时测定怀牛膝中3种甾酮类成分

Simultaneous determination of three sterones in Achyranthis bidentatae Radix by RP-HPLC

摘要: 建立怀牛膝中3种甾酮类成分含量测定方法，比较不同产地牛膝的质量差异。色谱柱为Venusil XBP C₁₈柱，流动相为0.1%甲酸-乙腈(84∶16)，检测波长为250 nm。在所建立的方法中，β-蜕皮甾酮、R-牛膝甾酮和S-牛膝甾酮与杂质分离良好，线性范围分别为3.07~1 470.00 μg/mL(r=0.999 4，n=6)，0.52~246.00 μg/mL(r=0.999 7，n=6)，0.47~225.00 μg/mL(r=0.999 8，n=6)，平均回收率分别为101.4%、101.1%和101.1%。本方法适用于同时测定怀牛膝中β-蜕皮甾酮、R-牛膝甾酮及S-牛膝甾酮的含量。

Abstract: An HPLC method was established for the simultaneous determination of β-ecdysterone, R-inokosterone and S-inokosterone in the Achyranthis bidentatae Radix to compare the quality from different habitats. Three phytoecdysones were separated with a Venusil XBP C₁₈ column by isocratic elution using 0. 1% formic acid in water and acetonitrile(84 ∶16)as the mobile phase, while the UV detector wavelength was set at 250 nm. Calibration curves of β-ecdysterone, R-inokosterone and S-inokosterone were linear over the range of 3. 07-1 470. 00, 0. 52-246. 00, and 0. 47-225. 00 μg/mL, respectively. Average recoveries were 101. 4%, 101. 1% and 101. 1%, respectively. The established method will be helpful in the quality control of Achyranthis bidentatae Radix.