HPLC法同时测定怀牛膝中3种甾酮类成分
Simultaneous determination of three sterones in Achyranthis bidentatae Radix by RP-HPLC
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摘要: 建立怀牛膝中3种甾酮类成分含量测定方法，比较不同产地牛膝的质量差异。色谱柱为Venusil XBP C18柱，流动相为0.1%甲酸-乙腈(84∶16)，检测波长为250 nm。在所建立的方法中，β-蜕皮甾酮、R-牛膝甾酮和S-牛膝甾酮与杂质分离良好，线性范围分别为3.07~1 470.00 μg/mL(r=0.999 4，n=6)，0.52~246.00 μg/mL(r=0.999 7，n=6)，0.47~225.00 μg/mL(r=0.999 8，n=6)，平均回收率分别为101.4%、101.1%和101.1%。本方法适用于同时测定怀牛膝中β-蜕皮甾酮、R-牛膝甾酮及S-牛膝甾酮的含量。Abstract: An HPLC method was established for the simultaneous determination of β-ecdysterone, R-inokosterone and S-inokosterone in the Achyranthis bidentatae Radix to compare the quality from different habitats. Three phytoecdysones were separated with a Venusil XBP C18 column by isocratic elution using 0. 1% formic acid in water and acetonitrile(84 ∶16)as the mobile phase, while the UV detector wavelength was set at 250 nm. Calibration curves of β-ecdysterone, R-inokosterone and S-inokosterone were linear over the range of 3. 07-1 470. 00, 0. 52-246. 00, and 0. 47-225. 00 μg/mL, respectively. Average recoveries were 101. 4%, 101. 1% and 101. 1%, respectively. The established method will be helpful in the quality control of Achyranthis bidentatae Radix.
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Keywords:
- Achyranthis bidentatae Radix /
- quantification /
- β-ecdysterone /
- R-inokosterone /
- S-inokosterone
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[1] Chinese Pharmacopoeia Commission.Chinese Pharmacopoeia:part 1(中华人民共和国药典:一部)[S].Beijing:China Medical Science Press,2010:67. [2] Shen S,Wang Q,Li YB.Studies on chemical constituents an pharmaceutics activity of Achyranthes bidentata Bl[J].Strait Pharma J(海峡药学),2011,23(11):1-6. [3] Sun FY,Pan QH,Hong A.The Effect of promotion osteoblast proliferation and mechanism research of Achyranthes[J].J Chin Mater Med(中药材),2004,27(4):264-265.
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