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源自桑树资源的10种酚类化合物的抑菌活性研究

庞道睿, 刘凡, 施英, 刘军, 沈维治, 邹宇晓, 廖森泰, 肖更生

庞道睿, 刘凡, 施英, 刘军, 沈维治, 邹宇晓, 廖森泰, 肖更生. 源自桑树资源的10种酚类化合物的抑菌活性研究[J]. 中国药科大学学报, 2014, 45(2): 221-226. DOI: 10.11665/j.issn.1000-5048.20140216
引用本文: 庞道睿, 刘凡, 施英, 刘军, 沈维治, 邹宇晓, 廖森泰, 肖更生. 源自桑树资源的10种酚类化合物的抑菌活性研究[J]. 中国药科大学学报, 2014, 45(2): 221-226. DOI: 10.11665/j.issn.1000-5048.20140216
PANG Daorui, LIU Fan, SHI Ying, LIU Jun, SHEN Weizhi, ZOU Yuxiao, LIAO Sentai, XIAO Gengsheng. Antibacterial activity of 10 phenolic compounds from mulberry[J]. Journal of China Pharmaceutical University, 2014, 45(2): 221-226. DOI: 10.11665/j.issn.1000-5048.20140216
Citation: PANG Daorui, LIU Fan, SHI Ying, LIU Jun, SHEN Weizhi, ZOU Yuxiao, LIAO Sentai, XIAO Gengsheng. Antibacterial activity of 10 phenolic compounds from mulberry[J]. Journal of China Pharmaceutical University, 2014, 45(2): 221-226. DOI: 10.11665/j.issn.1000-5048.20140216

源自桑树资源的10种酚类化合物的抑菌活性研究

基金项目: 国家自然科学基金资助项目(No.31071534);现代农业产业技术体系建设专项资金项目(No.CARS-22);广东省自然科学基金团队项目(No.9351064001000002);广州市珠江科技新星专项资金项目(No.2013J2200085)

Antibacterial activity of 10 phenolic compounds from mulberry

  • 摘要: 为了深入研究源自桑树资源的酚类化合物的抑菌活性,采用琼脂扩散法和二倍微量稀释法,测定了Morusin、Kuwanon E等10种桑树资源酚类化合物对金黄色葡萄球菌、枯草芽孢杆菌、大肠埃希菌和沙门氏菌4种常见致病菌的抑菌圈直径和最低抑菌浓度。结果表明:所测定化合物对革兰阳性菌(金黄色葡萄球菌、枯草芽孢杆菌)的抑菌效果普遍优于对革兰阴性菌(大肠埃希菌、沙门氏菌);所测定化合物中,Morusin、Kuwanon E和Sanggenol A的抑菌活性最强,其中Morusin对枯草芽孢杆菌的最低抑菌浓度最低(<;3.72 μmol/L)。对所测定化合物抑菌活性的构效关系进行了初步探讨,发现化合物的立体构象、取代基种类和取代位置对其抑菌活性均有影响,特别是异戊二烯基结构对化合物的抑菌活性影响最大。
    Abstract: Inhibition zone diameters and minimum inhibitory concentrations(MIC)of 10 phenolic compounds from mulberry(Morus alba L. )against 4 common pathogenic bacteria(Staphyloccocus aureus, Bacillus subtilis, Escherichia coli, Salmonella spp )were tested by agar diffusion and micro-dilution in order to study the antibacterial activity of mulberry. The results indicated that the monomeric compounds exhibited more effect on Gram-positive bacteria(Staphylococcus aureus, Bacillus subtilis)than Gram-negative ones(E. coli, Salmonella). The antibacterial activity of Morusin, Kuwanon E and Sanggenol A on Gram-positive bacteria were the best. Especially, the MIC of Morusin against Bacillus subtilis was below 3. 72 μmol/L. Structure-activity relationships of these compounds were also discussed. It was found that stereoisomerism, species and position of substituent had implications for their antibacterial activity. Especially, prenyl was very important for the antibacterial activity of compounds.
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  • 刊出日期:  2014-04-24

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