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染料木素对Aβ诱导损伤的PC12细胞的保护作用

赵美顺, 陈敬荣, 郑尧杰, 杨红

赵美顺, 陈敬荣, 郑尧杰, 杨红. 染料木素对Aβ诱导损伤的PC12细胞的保护作用[J]. 中国药科大学学报, 2014, 45(2): 227-231. DOI: 10.11665/j.issn.1000-5048.20140217
引用本文: 赵美顺, 陈敬荣, 郑尧杰, 杨红. 染料木素对Aβ诱导损伤的PC12细胞的保护作用[J]. 中国药科大学学报, 2014, 45(2): 227-231. DOI: 10.11665/j.issn.1000-5048.20140217
ZHAO Meishun, CHEN Jingrong, ZHENG Yaojie, YANG Hong. Protective effect of genistein on Aβ25-35-induced PC12 cells injury[J]. Journal of China Pharmaceutical University, 2014, 45(2): 227-231. DOI: 10.11665/j.issn.1000-5048.20140217
Citation: ZHAO Meishun, CHEN Jingrong, ZHENG Yaojie, YANG Hong. Protective effect of genistein on Aβ25-35-induced PC12 cells injury[J]. Journal of China Pharmaceutical University, 2014, 45(2): 227-231. DOI: 10.11665/j.issn.1000-5048.20140217

染料木素对Aβ诱导损伤的PC12细胞的保护作用

基金项目: 广东省科技计划项目资助(No.2012B031800430,No.2010B020312016)

Protective effect of genistein on Aβ25-35-induced PC12 cells injury

  • 摘要: 研究染料木素(genistein,Gen)对Aβ诱导损伤的PC12细胞的保护作用及机制。采用RT-PCR检测Gen及其拮抗剂(Myr)对PC12损伤细胞Bcl-2和Bax表达水平的影响,Hoechst 33342/PI双染检测细胞凋亡率的改变,并检测Gen及Myr对蛋白激酶C(protein kinase C,PKC)活性的影响。实验结果表明,Gen能上调Aβ诱导损伤的PC12细胞Bcl-2基因和下调Bax基因的表达水平,增加Bcl-2/Bax的比值,降低细胞的凋亡程度,提高PKC活性,该作用被其拮抗剂Myr所抑制。Gen对Aβ诱导的PC12损伤细胞具有保护作用,其机制是通过激活PKC信号通路,调节Bcl-2/Bax凋亡基因表达,从而减少细胞损伤。
    Abstract: To investigate the inhibitory effect of Genistein(Gen)on Aβ25-35-induced neurotoxicity and to elucidate the underlying mechanism in cultured rat pheochromocytoma(PC12)cells. The injured PC12 cells were treated with Gen and its antagonist, Myr. The influence on the expression of Bcl-2 and Bax mRNA levels was detected by RT-PCR. Cell viability was assessed by staining with Hoechst 33342/PI. Protein kinase C(PKC)activity was measured with the intervention of Gen and PKC inhibitor(Myr). The results showed that Gen could increase the Bcl-2 expression, decrease the Bax expression, increase the ratio of Bcl-2/Bax, and significantly increase cell viability, as well as the activity of PKC in Aβ25-35-treated PC12 cells. Myr, a PKC inhibitor, partially blocked the activation effect of Gen. Gen exerted protective effect on Aβ25-35-induced neurotoxicity via activating the PKC signaling pathway, which further regulated the Bcl-2/Bax expression.
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出版历程
  • 刊出日期:  2014-04-24

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