• 中国中文核心期刊
  • 中国科学引文数据库核心期刊
  • 中国科技核心期刊
  • 中国高校百佳科技期刊
高级检索

丹酚酸A对急性心肌缺血大鼠尿酸代谢的影响

李茜, 何华, 刘瑶, 王世俊, 王海东, 柳晓泉

李茜, 何华, 刘瑶, 王世俊, 王海东, 柳晓泉. 丹酚酸A对急性心肌缺血大鼠尿酸代谢的影响[J]. 中国药科大学学报, 2014, 45(3): 335-340. DOI: 10.11665/j.issn.1000-5048.20140315
引用本文: 李茜, 何华, 刘瑶, 王世俊, 王海东, 柳晓泉. 丹酚酸A对急性心肌缺血大鼠尿酸代谢的影响[J]. 中国药科大学学报, 2014, 45(3): 335-340. DOI: 10.11665/j.issn.1000-5048.20140315
shu
LI Xi, HE Hua, LIU Yao, WANG Shijun, WANG Haidong, LIU Xiaoquan. Effect of salvianolic acid A on uric acid metabolism in acute myocardial infarction rats[J]. Journal of China Pharmaceutical University, 2014, 45(3): 335-340. DOI: 10.11665/j.issn.1000-5048.20140315
Citation: LI Xi, HE Hua, LIU Yao, WANG Shijun, WANG Haidong, LIU Xiaoquan. Effect of salvianolic acid A on uric acid metabolism in acute myocardial infarction rats[J]. Journal of China Pharmaceutical University, 2014, 45(3): 335-340. DOI: 10.11665/j.issn.1000-5048.20140315
shu

丹酚酸A对急性心肌缺血大鼠尿酸代谢的影响

  • 中国药科大学药物代谢动力学研究中心

基金项目: 国家自然科学基金资助项目(No.81273588)

Effect of salvianolic acid A on uric acid metabolism in acute myocardial infarction rats

摘要

    摘要
  • 摘要: 通过结扎大鼠冠状动脉左前降支建立急性心肌缺血大鼠模型，考察丹酚酸A对急性心肌缺血大鼠尿酸代谢的影响。大鼠随机分为3组，分别为假手术组、结扎组及丹酚酸A组(5 mg/kg)。测定血浆及心肌组织中尿酸含量，同时测定血浆黄嘌呤氧化酶活性，心肌组织中高能磷酸化合物及嘌呤化合物含量，以及心肌组织缺血率。结果表明，急性心肌缺血发生后，心肌组织中ATP耗竭，心肌组织缺血率增加，细胞坏死嘌呤化合物释出进入循环系统，心肌组织中嘌呤化合物减少。血浆中尿酸底物及黄嘌呤氧化酶活性增加，共同造成血浆尿酸水平升高。丹酚酸A可增加高能磷酸化合物及嘌呤化合物含量，减小心肌缺血率，减少细胞坏死，同时抑制血浆中黄嘌呤氧化酶活性，从两个方面减少血浆中尿酸生成。提示丹酚酸A可通过调节尿酸的生成发挥心血管保护作用。
    Abstract: The aim of this study was to investigate the effect of salvianolic acid A(SalA)on uric acid metabolism in acute myocardial infarction(AMI)rats. Rats were randomly divided into Sham, AMI and SalA groups. The AMI model underwent coronary artery ligation and the survival AMI rats received a single intravenous dose of 5 mg/kg of SalA and normal saline. The uric acid plasma and myocardial concentrations, myocardial adenine nucleotides(AMP, ADP and ATP), inosine, xanthine, hypoxanthine were determined by HPLC and plasma xanthine oxidase activity was measured spectrophotometrically using commercial diagnostic kits. Myocardial infarct rate was measured by TTC dying method. After AMI, myocardial ATP depleted, the infarct rate increased and purine compounds released from necrotic cells, resulting in the increased uric acid substrate in plasma. Furthermore, the xanthine oxidase activity in plasma was increased, so the elevated substrate and increased xanthine oxidase activity resulted in elevated plasma uric acid levels. SalA increased high-energy phosphate compounds and inosine levels, reduced myocardial infarct rate, so less purine compound released to plasma. SalA also inhibited xanthine oxidase activity in plasma. Therefore, plasma uric acid was declined. The results suggested that SalA could exhibit cardiovascular protective effects by regulating of uric acid synthesis in AMI rats.
    • HTML全文
    • 参考文献(27)
  • [1] Chan D,Ng LL.Biomarkers in acute myocardial infarction[J].BMC Med,2010,8(1):34.
    [2] Kanbay M,Segal M,Afsar B,et al.The role of uric acid in the pathogenesis of human cardiovascular disease[J].Heart,2013,99(11):759-766.
    [3] Lee BE,Toledo AH,Anaya-Prado R,et al.Allopurinol,xanthine oxidase,and cardiac ischemia[J].J Investig Med,2009,57(8):902-909.
    [4] de Oliveira EP,Burini RC.High plasma uric acid concentration:causes and consequences[J].Diabetol Metab Syndr,2012,4(1):12.
    [5] Lazaros G,Tsiachris D,Aznaouridis K,et al.Uric acid in-hospital changes predict mortality in patients with acute myocardial infarction[J].Nutr Metab Cardiovasc Dis,2013,23(12):1 202-1 209.
    [6] Sedaghat S,Hoorn EJ,van Rooij FJ,et al.Serum uric acid and chronic kidney disease:the role of hypertension[J].PloS One,2013,8(11):e76827.
    [7] Xu Y,Zhu J,Gao L,et al.Hyperuricemia as an independent predictor of vascular complications and mortality in type 2 diabetes patients:a meta-analysis[J].PloS one,2013,8(10):e78206.
    [8] Levantesi G,Marfisi RM,Franzosi MG,et al.Uric acid:a cardiovascular risk factor in patients with recent myocardial infarction[J].Int J Cardiol,2013,167(1):262-269.
    [9] Fan H,Fu F,Yang M,et al.Antiplatelet and antithrombotic activities of salvianolic acid A[J].Thromb Res,2010,126(1):e17-e22.
    [10] Wang S,Tian S,Yang F,et al.Cardioprotective effect of salvianolic acid A on isoproterenol-induced myocardial infarction in rats[J].Eur J Pharmacol,2009,615(1):125-132.
    [11] Sun L,Zhao R,Zhang L,et al.Salvianolic acid A inhibits PDGF-BB induced vascular smooth muscle cell migration and proliferation while does not constrain endothelial cell proliferation and nitric oxide biosynthesis[J].Molecules,2012,17(3):3 333-3 347.
    [12] Yang L,Li D,Zhang Y,et al.Salvianolic acid A inhibits angiotensin II-induced proliferation of human umbilical vein endothelial cells by attenuating the production of ROS[J].Acta Pharmacol Sin,2011,33(1):41-48.
    [13] Zhang L,Chang BB,Chao WW,et al.Effect of danshensu on myocardial energy metabolism in isolated ischemia reperfusion rat hearts[J].J China Pharm Univ(中国药科大学学报),2010,41(3):278-282.
    [14] Huang LH,Chen YC,Cheng Y,et al.Effect of content variation of water-soluble components on myocardial energy metabolism in isolated ischemia-reperfusion rat hearts[J].J China Pharm Univ(中国药科大学学报),2011,42(4):348-353.
    [15] Ma C,Huo SF,Cao YG,et al.Regulation of Danshen injection on cardiac energy metabolism in rats[J].J China Pharm Univ(中国药科大学学报),2009,40(2):147-151.
    [16] Carrasco-Pozo C,Gotteland M,Speisky H.Apple peel polyphenol extract protects against indomethacin-induced damage in Caco-2 cells by preventing mitochondrial complex I inhibition[J].J Agric Food Chem,2011,59(21):11 501-11 508.
    [17] Bagul PK,Middela H,Matapally S,et al.Attenuation of insulin resistance,metabolic syndrome and hepatic oxidative stress by resveratrol in fructose-fed rats[J].Parmacol Res,2012,66(3):260-268.
    [18] Chen YC,Cao WW,Cao Y,et al.Using neural networks to determine the contribution of danshensu to its multiple cardiovascular activities in acute myocardial infarction rats[J].J Ethnopharmacol,2011,138(1):126-134?监ぢ????戱??㈠??扬???ラ??????????????oga P,et al.Development of an HPLC method for determination of metabolic compounds in myocardial tissue[J].J Pharm Biomed Anal,2004,35(3):647-653.
    [19] Akula KK,Kaur M,Bishnoi M,et al.Development and validation of an RP-HPLC method for the estimation of adenosine and related purines in brain tissues of rats[J].J Sep Sci,2008,31(18):3 139-3 147.
    [20] Coolen EJ,Arts IC,Swennen EL,et al.Simultaneous determination of adenosine triphosphate and its metabolites in human whole blood by RP-HPLC and UV-detection[J].J Chromatogr B Analyt Technol Biomed Life Sci,2008,864(1/2):43-51.
    [21] Wang J,Jiang Z,Ji J,et al.Evaluation of hepatotoxicity and cholestasis in rats treated with EtOH extract of Fructus Psoraleae[J].J Ethnopharmacol,2012,144(1):73-81.
    [22] Al-Shamiri SA,Hasan NA,Frankul WM,et al.Purines and oxypurines in myocardial ischemia[J].Saudi Med J,2009,30(2):257-266.
    [23] Lewis GD,Wei R,Liu E,et al.Metabolite profiling of blood from individuals undergoing planned myocardial infarction reveals early markers of myocardial injury[J].J Clin Invest,2008,118(10):3 503-3 512.
    [24] Ponce A,Blanco S,Molina A,et al.Study of the action of flavonoids on xanthine-oxidase by molecular topology[J].J Chem Inf Comput Sci,2000,40(4):1 039-1 045.
    [25] Sezai A,Soma M,Nakata K,et al.Comparison of febuxostat and allopurinol for hyperuricemia in cardiac surgery patients(NU-FLASH Trial)[J].Circ J,2013,77(8):2 043-2 049.
    [26] Halliwell B.Are polyphenols antioxidants or pro-oxidants? What do we learn from cell culture and in vivo studies[J]?Arch Biochem Biophys,2008,476(2):107-112.
    [27] He H,Wang S,Li X,et al.A novel metabolic balance model for describing the metabolic disruption of and interactions between cardiovascular-related markers during acute myocardial infarction[J].Metabolism
    • 相关文章
    • 施引文献
    • 资源附件(0)
计量
  • 文章访问数: 
  • HTML全文浏览量:  0
  • PDF下载量: 
  • 被引次数: 0
出版历程
  • 刊出日期:  2014-06-24

目录

摘要
HTML全文
    参考文献(27)
    相关文章
    施引文献
    资源附件(0)

    /

    返回文章
    返回

    版权所有：《中国药科大学学报》编辑部苏ICP备05007142号

    地址：江苏省南京市鼓楼区童家巷24号（210009）电话: 025-83271566E-mail: xuebao@cpu.edu.cn

    本系统由 北京仁和汇智信息技术有限公司 开发  百度统计

    访问量：335901

    x 关闭 永久关闭