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氢化咖啡酰壳聚糖-铜-阿霉素三元配位超分子的制备及肾靶向评价

Preparation and characterization of hydrocaffeoylchitosan-copper-doxorubicin ternary coordination supermolecule for renal targeting

  • 摘要: 合成了氢化咖啡酰壳聚糖衍生物(HCAC),其中氢化咖啡酸(HCA)的取代度为15.6%;采用不良溶剂沉淀法将HCAC、Cu(NO3)2·3H2O和阿霉素(Dox)制备成三元配位超分子HCAC-Cu-Dox。通过高效液相色谱(HPLC)和电感耦合等离子体原子发射光谱(ICP-AES)分别测定Dox和Cu的含量为(7.8±0.3)%和(5.2±0.2)%;透射电镜(TEM)观察超分子为类球形纳米粒子,平均粒径为(68.7±3.9)nm;研究了超分子的键合常数和热力学性质,证明Dox在超分子中以分子态或无定型态存在;体外释放结果显示载药超分子在生理pH条件下无突释现象,24 h释放量仅13.5%;近红外荧光成像表明超分子可有效分布至肾,而极少在其他脏器分布;MTT法研究超分子的细胞毒性,表明其可明显抑制人肾癌A498细胞的增殖。因此,本研究所制备的HCAC-Cu-Dox三元配位超分子有望实现肾靶向递药和治疗。

     

    Abstract: Hydrocaffeoylchitosan(HCAC)was synthesized and characterized with 15. 6% substitution degree of hydrocaffeic acid. Ternary coordination supermolecule HCAC-Cu-Dox was subsequently fabricated through the solvent-induced precipitation method in the presence of HCAC, Cu(NO3)2 ·3H2O and Dox. The contents of Dox and Cu were(7. 8±0. 3)% and(5. 2±0. 2)% determined by high-performance liquid chromatography(HPLC)and inductively coupled plasma-atomic emission spectrometry(ICP-AES), respectively. The supermolecule was in spherical shape with an average particle size of(68. 7±3. 9)nm observed by TEM. The binding constant of supermolecule was measured, and thermodynamic property was evaluated, indicating that Dox installed in supermolecule existed in amorphous or molecular state. Besides, in vitro release profile indicated that supermolecule possessed satisfactory stable in physiological conditions, and only 13. 5% of Dox were detected in 24 h. Furthermore, the near-infrared fluorescence(NIRF)imaging showed that the supermolecule could be specifically delivered to kidney after intravenous injection, while hardly accumulated in other organs. Cytotoxicity assay by MTT method showed that A498 cells were significantly inhibited after incubation with HCAC-Cu-Dox. In conclusion, HCAC-Cu-Dox ternary coordination supermolecule has potential for renal targeting and treatment.

     

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