Abstract: A UPLC method was established for the simultaneous determination of protocatechuic acid, chlorogenic acid, pumiloside, 3-epi-pumiloside, strictosamide and vincosamide in Nauclea officinalis. The experiment was performed with an Acquity BEH C₁₈(2. 1 mm ×100 mm, 1. 7 μm)column and the mobile phase consisting of acetonitrile and 0. 1% formic acid with gradient elution. The flow rate of 0. 4 mL/min and the injection volume of 2 μL as well as the column temperature of 40 ℃ were employed. Under the above-mentioned condition, 6 components in Nauclea officinalis were separated completely and the method was fully validated. Good linearity(R²> 0. 999 6), precision of inter- and intra-day(RSD≤4. 05%)together with average recoveries of 96. 59%-101. 8% were obtained. The method was proved to be rapid and accurate and can be used for the quality control of Nauclea officinalis.