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UPLC法同时测定胆木中6种成分的含量

陈家全, 徐光富, 王慧, 王静静, 朱粉霞

陈家全, 徐光富, 王慧, 王静静, 朱粉霞. UPLC法同时测定胆木中6种成分的含量[J]. 中国药科大学学报, 2014, 45(4): 434-437. DOI: 10.11665/j.issn.1000-5048.20140409
引用本文: 陈家全, 徐光富, 王慧, 王静静, 朱粉霞. UPLC法同时测定胆木中6种成分的含量[J]. 中国药科大学学报, 2014, 45(4): 434-437. DOI: 10.11665/j.issn.1000-5048.20140409
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CHEN Jiaquan, XU Guangfu, WANG Hui, WANG Jingjing, ZHU Fenxia. Simultaneous determination of six main compounds in Nauclea officinalis by ultra-performance liquid chromatography[J]. Journal of China Pharmaceutical University, 2014, 45(4): 434-437. DOI: 10.11665/j.issn.1000-5048.20140409
Citation: CHEN Jiaquan, XU Guangfu, WANG Hui, WANG Jingjing, ZHU Fenxia. Simultaneous determination of six main compounds in Nauclea officinalis by ultra-performance liquid chromatography[J]. Journal of China Pharmaceutical University, 2014, 45(4): 434-437. DOI: 10.11665/j.issn.1000-5048.20140409
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UPLC法同时测定胆木中6种成分的含量

  • 1.

    中国药科大学分析化学教研室

  • 2.

    江苏省中医药研究院国家中医药管理局中药口服制剂释药系统重点研究室

基金项目: 国家自然科学基金资助项目(No.81203001)

Simultaneous determination of six main compounds in Nauclea officinalis by ultra-performance liquid chromatography

摘要

    摘要
  • 摘要: 建立胆木药材中原儿茶酸、绿原酸、短小舌根草苷、3-表短小舌根、异常春花苷内酰胺和喜果苷同时测定的UPLC方法。采用Acquity BEH C18色谱柱(2.1 mm×100 mm，1.7 μm)，乙腈-0.1%甲酸为流动相，梯度洗脱，流速为0.4 mL/min，进样量为2 μL，柱温为40 ℃的色谱条件。在选定的色谱条件下，6种成分均能达到良好分离，并对方法进行了系统的验证。结果表明，线性关系良好(R2>；0.999 6)，日内、日间精密度RSD≤ 4.05%，加样回收率为96.59%~101.8%。本方法简便、快速、准确，可用于胆木药材的质量控制。
    Abstract: A UPLC method was established for the simultaneous determination of protocatechuic acid, chlorogenic acid, pumiloside, 3-epi-pumiloside, strictosamide and vincosamide in Nauclea officinalis. The experiment was performed with an Acquity BEH C18(2. 1 mm ×100 mm, 1. 7 μm)column and the mobile phase consisting of acetonitrile and 0. 1% formic acid with gradient elution. The flow rate of 0. 4 mL/min and the injection volume of 2 μL as well as the column temperature of 40 ℃ were employed. Under the above-mentioned condition, 6 components in Nauclea officinalis were separated completely and the method was fully validated. Good linearity(R2> 0. 999 6), precision of inter- and intra-day(RSD≤4. 05%)together with average recoveries of 96. 59%-101. 8% were obtained. The method was proved to be rapid and accurate and can be used for the quality control of Nauclea officinalis.
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    • 参考文献(10)
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    [2] Xuan WD.Studies on bio-active constituents of Nauclea officinalis and Ervatamia Yunnanensis(中药胆木和云南狗牙花活性成分研究)[D].Shanghai:Second Military Medical University,2005.
    [3] Tao JY.Studies on Chemical components of Nauclea officinalis(药乌檀化学成分的研究)[D].Shengyang:Shengyang Pharmaceautical University,2007.
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  • 刊出日期:  2014-08-24

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