UPLC法同时测定胆木中6种成分的含量
Simultaneous determination of six main compounds in Nauclea officinalis by ultra-performance liquid chromatography
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摘要: 建立胆木药材中原儿茶酸、绿原酸、短小舌根草苷、3-表短小舌根、异常春花苷内酰胺和喜果苷同时测定的UPLC方法。采用Acquity BEH C18色谱柱(2.1 mm×100 mm,1.7 μm),乙腈-0.1%甲酸为流动相,梯度洗脱,流速为0.4 mL/min,进样量为2 μL,柱温为40 ℃的色谱条件。在选定的色谱条件下,6种成分均能达到良好分离,并对方法进行了系统的验证。结果表明,线性关系良好(R2>;0.999 6),日内、日间精密度RSD≤ 4.05%,加样回收率为96.59%~101.8%。本方法简便、快速、准确,可用于胆木药材的质量控制。Abstract: A UPLC method was established for the simultaneous determination of protocatechuic acid, chlorogenic acid, pumiloside, 3-epi-pumiloside, strictosamide and vincosamide in Nauclea officinalis. The experiment was performed with an Acquity BEH C18(2. 1 mm ×100 mm, 1. 7 μm)column and the mobile phase consisting of acetonitrile and 0. 1% formic acid with gradient elution. The flow rate of 0. 4 mL/min and the injection volume of 2 μL as well as the column temperature of 40 ℃ were employed. Under the above-mentioned condition, 6 components in Nauclea officinalis were separated completely and the method was fully validated. Good linearity(R2> 0. 999 6), precision of inter- and intra-day(RSD≤4. 05%)together with average recoveries of 96. 59%-101. 8% were obtained. The method was proved to be rapid and accurate and can be used for the quality control of Nauclea officinalis.
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Keywords:
- Nauclea officinalis /
- chemical constituents /
- determination of content /
- UPLC
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[1] Editorial Committee of Chinese Herbs.Chinese Herbs(中华本草)[M].Shanghai:Shanghai Science and Technology Press,1999:4 561. [2] Xuan WD.Studies on bio-active constituents of Nauclea officinalis and Ervatamia Yunnanensis(中药胆木和云南狗牙花活性成分研究)[D].Shanghai:Second Military Medical University,2005. [3] Tao JY.Studies on Chemical components of Nauclea officinalis(药乌檀化学成分的研究)[D].Shengyang:Shengyang Pharmaceautical University,2007. [4] Hu X.Nauclea officinalis:Chemical components and pharmacological effects(乌檀化学成分分离分析与相关成分活性、药动学研究)[D].Shengyang:Shengyang Pharmaceautical University,2007. [5] Wang JJ.The chemical constituents and material basis investigation of Nauclea officinalis on anti-inflammatort effects(胆木注射液化学成分及其抗炎物质基础研究)[D].Zhenjiang:Jiangsu University,2012. [6] Zhu FX,Chen JQ,Wang JJ,et al.Qualitative and quantitative analysis of the constituents in Danmu preparations by UPLC/PDA-TOF-MS[J].J Chromatogr Sci,2013,doi: 10.1093/chromsci/bmt129. [7] Zhu FX,Jia XB,Li XF,et al.Simultaneous determination of protocatechuic acid,neochlorogenic acid,chlorogenic acid and cryptochlorogenic acid in Danmu injection by UPLC[J].China Tradit Herb Drugs(中草药),2013,44(5):571-573. [8] Zhao Y,Zhao J,Li XP,et al.Advances in caffeoylquinic acid research[J].Chin J Chin Materia Medica(中国中药杂志),2006,31(11):869-874. [9] Zhang AL,Ma Q,Gao JM.Studies on bioactivities of chlorogenic acid and its analogues[J].China Tradit Herb Drugs(中草药),2001,32(2):173-176. [10] Zhu FX,Wang JJ,Song J,et al.Chemical constituents of Nauclea officinalis[J].Acta Pharm Sin(药学学报),2013,48(2):276-280. -
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