Abstract: To investigate the anti-metastasis effect of wogonin on human alveolar adenocarcinoma cell A549 induced by IL-6 and its potential mechanism, the growth inhibition effect of wogonin by MTT assay and the anti-metastasis effect of wogonin by transwell invasion assay were investigated. The immunofluorescence and Real-time PCR assay were employed to observe wogonin regulating metastasis marker protein E-cadherin, N-cadherin, Vimentin protein and mRNA level expression. Snail and Twist mRNA level expression and transcription factors of metastasis were also detected. In order to explore the potential mechanism of wogonin, PcDNA3. 1-Flag-STAT3 was transfected into A549 to detect metastasis marker protein mRNA level expression. Data showed that wogonin inhibited A549 cell invasion and metastasis, and regulated metastasis-related transcription factors and metastasis marker protein expression in a dose-dependent manner. In conclusion, all these results showed that wogonin can suppress A549 cells invasion andmetastasis induced by IL-6. It is possible that wogonin inhibits A549 cell invasion and metastasis by regulating the activity of transcription factor Snail and Twist.