Abstract: In recent years, techeniqus as zinc-finger nucleases(ZFNs), transcription activator-like effector nucleases(TALENs)and clustered regulatory interspaced short palindromic repeats CRISPR/Cas-based RNA-guided DNA endonucleases(CRISPR/Cas)have been applied for genomics editing scale. According to their high efficiency and customizable possibility, such techeniques have a significant influence upon the following research aspects, gene therapy, cell model, protein glycoengineer technology, cell engineering technology, and ect. , which have also been promoting several novel strategies and study methods involved rapidly. This review summerizes the development of genome editing technology and its practical application inside the field of gene therapy and biophamarceutical industry.