Abstract:
Lovastatin-loaded nanostructured lipid carrier(LT-NLC)was prepared by lovastatin(LT)and the lipid components of high density lipid protein(HDL). The physicochemical parameters of LT-NLC were characterized in terms of morphology, particle size, Zeta potential, entrapment efficiency, and crystallization behavior. The LT-NLC-apoprotein(LT-NLC-apo), represented effective form
in vivo, was prepared by incubating LT-NLC with native HDL. The escaping ability from macrophage cells phagocytosis and targeting effect to foam cells were evaluated, and the pharmacokinetic behavior was invesigated on rabbits. The results showed that LT-NLC was solid spherical or oval in shape with an average diameter of(14. 6±2. 0)nm, Zeta potential of(-32. 3±0. 4)mV and entrapment efficiency of(96. 2±1. 3)%. Phagocytosis studies showed that endogenous LT-NLC-apo can escape phagocytosis and target to foam cells. Pharmacokinetic data also prove that LT-NLC can escape phagocytosis effectively and extend the circulating time
in vivo. The study indicated that non-protein NLC-resembling HDL could be a useful tool to deliver lipophilic anti-atherogenic drugs to foam cells.