Abstract: In this study, a novel silica source named M-bionl was synthesized by(3-isocyanatopropyl)-triethoxysilane(ICPTES)and R-(+)-1, 1′-bi-2-naphthol. A new mesoporous silica was first obtained by M-bionl and Bis(triethoxysilyl)ethane(BTSE)as co-silica source, hexadecyl trimethyl ammonium bromide(CTAB)and PEO-PPO-PEO(P123)as co-template in acid condition. The diameter of particles was 3-5 μm with mean pore size of 5 nm. Then the separation capacity of the mesoporous silicas was tested, with excellent mechanical strength and complete separation of 5 kinds of binaphthyl compounds being achieved within 15 min, which indicated a promising application of these mesoporous silicas in HPLC and their separation performance was superior to commercial silica for binaphyhyl compounds.