Abstract:
The aim of the current study was to develop the methods for simultaneous determination of sodium danshensu, protocatechuic aldehyde, salvianolic acid B and puerarin in
Xinkeshu capsules by HPLC and UPLC. HPLC was performed on a Thermo C
18 column(4. 6 mm×250 mm, 5 μm)with a flow rate of 1. 0 mL/min. UPLC was performed on an Acquity UPLC T3 C
18 column(2. 1 mm×100 mm, 1. 8 μm)with a flow rate of 0. 5 mL/min. The mobile phases were both acetonitrile(A)-0. 1% trifluoroacetic acid solution(B)with gradient elution. The column temperatures were both 30 °C and the detection wavelengths were both set at 287 nm. Sodium danshensu, protocatechuic aldehyde, salvianolic acid B and puerarin were separated from adjacent peaks completely. The linear relationships of calibration curves were favorable. The recoveries(%,
n=9)of danshensu, protocatechuic aldehyde, salvianolic acid B and puerarin were 100. 9, 99. 4, 98. 9, 101. 4(HPLC)and 100. 3, 100. 6, 99. 1, 100. 7(UPLC), respectively. The content of the two methods for 4 components was consistent, but the procedure of gradient elution of UPLC was simpler than that of HPLC.