摘要:
建立高效液相色谱法(HPLC)、超高效液相色谱法(UPLC)同时测定心可舒胶囊中丹参素钠、原儿茶醛、丹酚酸B和葛根素的含量测定方法。HPLC法采用Thermo C18(4.6 mm×250 mm，5 μm)色谱柱，流速为1.0 mL/min；UPLC法采用Acquity T3 C18(2.1 mm×100 mm，1.8 μm)色谱柱，流速为0.5 mL/min，流动相均为乙腈-0.1%三氟乙酸水溶液，梯度洗脱，柱温均为30℃，检测波长均为287 nm。结果显示，丹参素钠、原儿茶醛、丹酚酸B和葛根素与相邻峰完全分离，线性关系良好，平均加样回收率(%，n=9)分别为100.9，99.4，98.9，101.4(HPLC)和100.3，100.6，99.1，100.7(UPLC)。两种方法测定的4种成分的含量一致，但UPLC法梯度洗脱程序较HPLC法简单。
Abstract:
The aim of the current study was to develop the methods for simultaneous determination of sodium danshensu, protocatechuic aldehyde, salvianolic acid B and puerarin in Xinkeshu capsules by HPLC and UPLC. HPLC was performed on a Thermo C18 column(4. 6 mm×250 mm, 5 μm)with a flow rate of 1. 0 mL/min. UPLC was performed on an Acquity UPLC T3 C18 column(2. 1 mm×100 mm, 1. 8 μm)with a flow rate of 0. 5 mL/min. The mobile phases were both acetonitrile(A)-0. 1% trifluoroacetic acid solution(B)with gradient elution. The column temperatures were both 30 °C and the detection wavelengths were both set at 287 nm. Sodium danshensu, protocatechuic aldehyde, salvianolic acid B and puerarin were separated from adjacent peaks completely. The linear relationships of calibration curves were favorable. The recoveries(%, n=9)of danshensu, protocatechuic aldehyde, salvianolic acid B and puerarin were 100. 9, 99. 4, 98. 9, 101. 4(HPLC)and 100. 3, 100. 6, 99. 1, 100. 7(UPLC), respectively. The content of the two methods for 4 components was consistent, but the procedure of gradient elution of UPLC was simpler than that of HPLC.
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