银杏内酯B mPEG-PLA聚合物胶束的制备和体外性质

Preparation and in vitro characterization of ginkgolide B-loaded mPEG-PLA polymeric micelles

摘要: 以聚乙二醇单甲醚-聚丙交酯嵌段共聚物(mPEG-PLA)(50/50)作为载体，制备银杏内酯B(GB)聚合物胶束(GB-mPEG-PLA)。采用乳化-溶剂挥发制备GB-mPEG-PLA溶液，再冷冻干燥保存，并对制得的GB-mPEG-PLA的载药量、包封率、粒径与分布和胶束形态等进行表征，采用透析法考察GB-mPEG-PLA的体外释放。所制得的GB-mPEG-PLA载药量为7.5%，包封率为82.2%，平均粒径为74.5 nm；扫描电镜显示胶束为类球形；GB-mPEG-PLA释放曲线显示其具有一定的缓释作用。研究结果表明，mPEG-PLA胶束是一种极具应用前景的纳米给药系统。

Abstract: Ginkgolide B-loaded mPEG-PLA polymeric micelles(GB-mPEG-PLA)was prepared by emulsification- solvent diffusion method. The drug loading, encapsulation efficiency, average particle size and size distribution of GB-mPEG-PLA were evaluated. Moreover, the in vitro release characteristics of GB-mPEG-PLA were investigated by dialysis. The average particle size of GB-mPEG-PLA was 74. 5 nm, the drug loading and encapsulation efficiency were 7. 5% and 82. 2%, respectively. SEM image showed GB-mPEG-PLA was spherical in shape. In addition, the release profile of ginkgolide B from GB-mPEG-PLA exhibited an extended-release pattern. Therefore, GB-mPEG-PLA can be a promising nano-sized drug delivery system in the future.