电导率测定法研究可注射有机凝胶的胶凝过程
Determination of electrical conductivity for the study of the gelling process of injectable organogel system
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摘要: 采用简便快捷的电导率测定法示踪可注射有机凝胶的体外胶凝过程，并通过对胶凝过程的模拟确定凝胶的胶凝时间及其影响因素，以获得具有适宜降解、释药行为的可注射有机凝胶。考察电导率测定法模拟胶凝过程的可行性，并评价凝胶因子类型和用量、抗凝剂种类和用量及温度等对胶凝过程的影响。结果显示:抗凝剂在介质中的剂量与电导率呈线性相关，测定体系电导率变化可示踪抗凝剂在胶凝过程中的扩散行为，并确定胶凝时间；凝胶因子结构及抗凝剂种类显著影响抗凝剂的扩散速率及扩散百分率，凝胶因子浓度增加和温度降低均可导致抗凝剂扩散速率减慢、胶凝时间延长，凝胶在2 h内可全部完成胶凝，提示凝胶突释结束、缓慢释药开始的时间为2 h。Abstract: The purpose of the present study was to develop a simple electrical conductivity-based determination method to trace the gelling process of injectable organogels after administration. Influential factors of gelling were firstly found in order to obtain an injectable organogel with suitable degradation and release behavior. The feasibility of conductivity measurement-based simulation of gelation process was studied, and the effects of the different kinds and amount of gelators and anti-gelling solvents as well as temperature on the gelling process were evaluated. Results indicated that the electrical conductivity of PBS solution was linearly proportional to the amount of anti-gelling solvent and that the change of the conductivity could trace the diffusion behavior of anti-gelling solvents in gelation process and obtain the gelation time. Structure of gelators and different kinds of anti-gelling solvents could observably affect the diffusion rate and diffusion percentage of anti-gelling solvent. The increase of N-stearoyl L-alanine methyl ester(SAM)and decrease of temperature could lead to the decrease of diffusion rate and extension of diffusion time. The gelation process of SAM organogel could be completed in two hours, indicating that the finishing time of sharpreleaseand the starting time of controlledrelease was two hours.
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Keywords:
- organogel /
- gelator /
- anti-gelling solvent /
- conductivity
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