益智仁中二苯基庚烷类化合物的血管生成抑制活性
Inhibitory effect of diarylheptanoids from Alpinia oxyphylla on angiogenesis
-
摘要: 探讨益智仁中两个已知二苯基庚烷类化合物Yakuchinone A和Yakuchinone B以及结构类似物姜黄素(curcumin)的血管生成抑制活性。采用了转基因荧光斑马鱼为体内实验动物模型，人脐静脉内皮细胞(HUVEC)为体外实验模型，评价了上述3个化合物的活性及毒性。以血管生成抑制指数(AI)代表化合物的活性与毒性之比。结果表明，无论是体外实验还是体内实验，姜黄素的血管生成抑制作用最强而毒性最低，Yakuchinone A次之，Yakuchinone B抑制活性最低而毒性最强。因此，姜黄素为三者中最佳的血管生成抑制剂。
-
关键词:
- 血管生成 /
- 斑马鱼 /
- 人脐静脉内皮细胞 /
- 姜黄素 /
- 二苯基庚烷化合物 /
- Yakuchinone A /
- Yakuchinone B
Abstract: This study investigated the anti-angiogenic activities of two diarylheptanoids, together with a structure analogue, curcumin. The activity and toxicity of these three compounds were compared using transgenic zebrafish as in vivo model and human umbilical vein endothelial cell(HUVEC)as in vitro model. Anti-angiogenic index(AI)was used as the ratio between LC50 and EC50. The results suggested that in both in vitro and in vivo assay, curcumin exerted the most potent anti-angiogenic effect but with lowest toxicity among these compounds; Yakuchinone A was the second potent; Yakuchinone B has the lowest activity but with the highest toxicity in all three compounds. Taken together, curcumin was the best angiogenic inhibitor in these three diarylheptanoids.-
Keywords:
- angiogenesis /
- zebrafish /
- HUVEC /
- curcumin /
- diarylheptanoid /
- Yakuchinone A /
- Yakuchinone B
-
-
[1] Finley SD, Chu LH, Popel AS. Computational systems biology approaches to anti-angiogenic cancer therapeutics[J].Drug Discov Today,2014.doi: 10.1016/j.drudis.2014.09.026. [2] Folkman J.Tumor angiogenesis:therapeutic implications[J].N Engl J Med,1971,285:1182-1186. [3] He MF,Huang YH,Wu LW,et al.Triptolide functions as a potent angiogenesis inhibitor[J].Int J Cancer,2010,126(1):266-278. [4] He ZH,He MF,Ma SC,et al.Anti-angiogenic effects of rhubarb and its anthraquinone derivatives[J].J Ethnopharmacol,2009,121(2):313-317. [5] Zhao D, Qin C, Fan X, et al. Inhibitory effects of quercetin on angiogenesis in larval zebrafish and human umbilical vein endothelial cells[J].Eur J Pharmacol,2014,723:360-367. [6] Alex D, Lam IK, Lin Z, et al. Indirubin shows anti-angiogenic activity in an in vivo zebrafish model and an in vitro HUVEC model[J].J Ethnopharmacol,2010,131(2):242-247. [7] Chun KS,Park KK,Lee J,et al.Inhibition of mouse skin tumor promotion by anti-inflammatory diaryheptanoids derived from Alpinia oxyphylla Miquel(Zingiberaceae)[J].Oncol Res,2002,13(1):37-45. [8] He ZH,Ge W,Yue GG,et al.Anti-angiogenic effects of the fruit of Alpinia oxyphylla[J].J Ethnopharmacol,2010,132(2):443-449. [9] Shoji N,Umeyama A,Takemoto T,et al.Isolation of a cardiotonic principle from Alpinia oxyphylla[J].Planta Med,1984,50(2):186-187. [10] Wang CM,Jing LJ,Wei YJ,et al.Evaluation of antiosteoporotic activity for micro-amount asperosaponins V and VI by on the osteoporosis model using zebrafish[J].J China Pharm Univ(中国药科大学学报),2014,45(1):88-91. [11] Liang D,Chang JR,Chin AJ,et al.The role of vascular endothelial growth factor(VEGF)in vasculogenesis,angiogenesis,and hematopoiesis in zebrafish development[J].Mech Dev,2001,108:29-43. [12] Pham VN, Roman BL, Weinstein BM, et al. Isolation and expression analysis of three zebrafish angiopoietin genes[J].Dev Dyn,2001,221(4):470-474. -
期刊类型引用(4)
1. 宋永真，李雅静，张振凌，王胜超. 曼地亚红豆杉酒炙工艺及质量标准研究. 中医学报. 2024(05): 1068-1075 . 
百度学术
2. 剡槿熙，臧明伍，刘贺，徐晨晨，史宇璇，白京，赵燕，郝静怡，张亚茹. 二萜类化合物在肉类食品保鲜防腐中的应用研究进展. 食品科学. 2024(13): 282-291 . 百度学术
3. 王楠楠，蔡婷婷，童晔玲，刘霞，朱婉萍，江石平，赵桂芝. 基于NF-κB信号通路的HTRF法筛选红豆杉中抗气道炎症有效活性成分研究. 浙江中医杂志. 2023(08): 553-556 . 百度学术
4. 傅贵江. 杉木人工林抚育间伐对套种南方红豆杉的影响. 武夷科学. 2022(01): 39-43 . 百度学术
其他类型引用(5)
计量
- 文章访问数: 1424
- HTML全文浏览量: 13
- PDF下载量: 2315
- 被引次数: 9
下载:
