雷公藤红素对白血病HL-60和Jurkat细胞增殖及凋亡的影响

张晓玲; 李爱萍; 环飞; 秦珩; 段金廒

doi:10.11665/j.issn.1000-5048.20150113

雷公藤红素对白血病HL-60和Jurkat细胞增殖及凋亡的影响

1.
南京医科大学公共卫生学院
2.
南京医科大学江苏省医药农药兽药安全性评价与研究中心
3.
南京中医药大学中药资源产业化与方剂创新药物国家地方联合工程研究中心

基金项目: 江苏高校优势学科建设工程资助项目

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- 刊出日期: 2015-02-24

Effects of celastrol on the proliferation and apoptosis of human leukemia cells HL-60 and Jurkat

摘要

摘要: 探讨雷公藤红素对人急性髓性白血病HL-60细胞、急性T淋巴细胞白血病Jurkat细胞增殖及凋亡的影响。采用MTT法、AnnexinV-FITC/PI双染法、PI染色法、透射电镜观察不同浓度雷公藤红素作用于两种细胞后对其生长增殖、凋亡、周期及形态等方面的影响。结果表明雷公藤红素能显著抑制HL-60细胞、Jurkat细胞的增殖，降低其存活率。给药24 h后，半抑制浓度(IC₅₀)分别为(0.46±1.05)μmol/L和(0.88±1.13)μmol/L。以剂量依赖方式诱导两种细胞凋亡，细胞周期分布G₁期比例增加，S期比例降低(P<；0.05)，且伴有典型的细胞凋亡形态学改变。雷公藤红素能显著抑制HL-60细胞、Jurkat细胞的生长增殖，并诱导细胞凋亡。
- 雷公藤红素 /
- HL-60细胞 /
- Jurkat细胞 /
- 凋亡
Abstract: To determine the effects of celastrol on the proliferation and apoptosis of cell lines HL-60 and Jurkat in human leukemia. Human leukemia cell lines were treated with celastrol at different concentrations. The inhibitory rate of cell proliferation was detected by MTT assay; the apoptosis rate was detected by AnnexinV-FITC/PI double staining; cell cycle was observed by PI staining; cell morphology was observed by transmission electron microscope(TEM). Cell proliferation was inhibited by celastrol, with IC₅₀ of(0. 46±1. 05)μmol/L and(0. 88±1. 13)μmol/L at 24 h. Celastrol induced cell apoptosis in a dose-dependent manner. The cell cycle distribution of G₁ phase rate increased, S phase rate decreased(P< 0. 05)with typical cell apoptosis-induced morphological changes. Results showed that celastrol could significantly inhibit cell proliferation and induce apoptosis in human leukemia cell lines of HL-60 and Jurkat.
- celastrol /
- HL-60 cell /
- Jurkat cell /
- apoptosis

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参考文献(11)

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雷公藤红素对白血病HL-60和Jurkat细胞增殖及凋亡的影响

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出版历程

Effects of celastrol on the proliferation and apoptosis of human leukemia cells HL-60 and Jurkat

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