替代对照品法同时测定前胡炮制品中主要香豆素成分
Simultaneous determination of major coumarins′ content in processing Peucedani Radix materials by method of substitute for reference substance
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摘要: 以白花前胡甲素为标准对照品，计算出白花前胡乙素和白花前胡E素的相对校正因子(f)，建立HPLC替代对照品法含量测定方法同时测定前胡药材中白花前胡甲素、乙素和E素含量，并在此基础上考察不同前胡炮制品中主要香豆素类成分的含量变化，为前胡药材及炮制品质量标准的制定及其临床应用提供科学依据。采用Welch Materials Ultimate XB-C18色谱柱(4.6 mm×250 mm，5 μm)，以甲醇-水为流动相，梯度洗脱，流速1.0 mL/min，柱温30 ℃，检测波长322 nm。该条件下测得前胡中白花前胡乙素和白花前胡E素相对于白花前胡甲素的相对校正因子分别为0.787 2和0.969 0，通过对15批药材进行含量测定，结果证明，替代对照品法与外标法测定的结果无明显差异，说明本研究建立的替代对照品法测定含量，方法准确可靠，可用于前胡及其不同炮制品中主要香豆素的含量测定。用该法测定各炮制品中香豆素总含量均低于生前胡。Abstract: (±)-Praeruptorin A(PA)was used as the standard reference substance to establish a new method of substitute for reference substance. The contents of both(±)-praeruptorin B(PB)and(+)-praeruptorin E(PE)were calculated by the method. The major content of coumarins in different processing materials could be gained by determining the content of(±)-praeruptorin A, which could provide scientific evidence for the research on pharmacology discrepancy and quality standards. The method was carried out on a Welch Materials Ultimate XB-C18 column(4. 6 mm×250 mm, 5 μm), with a gradient mobile phase of methanol and water at the flow rate of 1. 0 mL/min. The column temperature was 30 °C and detection wavelength was set at 322 nm. According to this method, the relative correlation factors(f)of(±)-praeruptorin B and(+)-praeruptorin E were determined as 0. 787 2 and 0. 969 0, respectively. After determine the contents of 15 batches of materials from different sources, no significant differences between substitute method and external standard method were observed. It was a feasible and credible method to determine the content of major coumarins in Peucedani Radix with different processing materials. The results showed lower contents of coumarins in processing materials than in raw materials.
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[1] Chinese Pharmacopoeia Commission.Chinese Pharmacopoeia:Part 1(中华人民共和国药典:一部)[S].Beijing:China Medical Science Press,2010:152. [2] Xuan ZH.UV spectrophotometry of total coumarin in Radix Peucedani[J].Chin Tradit Herb Drugs(中草药),2008,39(11):1741-1742. [3] Xu Z,Wang XB,Dai Y,et al.(±)-Praeruptorin A enantiomers exert distinct relaxant effects on isolated rat aorta rings dependent on endothelium and nitric oxide synthesis[J].Chem-Biol Interact,2010, 186(2):239-246. [4] Liang YM,Yu NJ,Liu SJ,et al.Optimum processing technology honey roast Peucedani[J].Chin Tradit Pat Med(中成药),2007, 29(3):399-401. [5] Wang XP,LI D,Long KH,et al.A quantitative method for simultaneous assay of three bioactive ingredients with one marker in Diphylleia sinensis Li[J].J Pharm Anal(药物分析杂志),2014, 34(8):1426-1430. [6] Cai XP,Li ZH,Hua JL,et al.Determination of six triterpene acid contents in effective fraction of Eriobotryae Folium by quantitative analysis of multi-components by single-marker[J].Chin Tradit Herb Drugs(中草药),2013, 44(21):3057-3062. [7] Yang F,Wan L,Hu YC,et al.Simultaneous determination of three coumarins in Angelica dahurica var.formosana by QAMS[J].Chin J Chin Mater Med(中国中药杂志),2012, 37(7):956-960. [8] Wang ZM,Qian ZZ,Zhang QW,et al.A guide to establish a multi-measurement technology assessment method[J].Chin J Chin Mater Med(中国中药杂志),2011,36(6):657-658. [9] Han C.Studies on process of extraction-purification and fingerprint of Peucedanum praeruptorum[D].Wuhan:Hubei University of Chinese Medicine,2011.
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