• 中国中文核心期刊
  • 中国科学引文数据库核心期刊
  • 中国科技核心期刊
  • 中国高校百佳科技期刊
高级检索

碳纳米粒的制备及其用于氯霉素的测定

严拯宇, 舒娟, 余雁, 张正伟, 唐璐, 陈建秋

严拯宇, 舒娟, 余雁, 张正伟, 唐璐, 陈建秋. 碳纳米粒的制备及其用于氯霉素的测定[J]. 中国药科大学学报, 2015, 46(3): 322-327. DOI: 10.11665/j.issn.1000-5048.20150310
引用本文: 严拯宇, 舒娟, 余雁, 张正伟, 唐璐, 陈建秋. 碳纳米粒的制备及其用于氯霉素的测定[J]. 中国药科大学学报, 2015, 46(3): 322-327. DOI: 10.11665/j.issn.1000-5048.20150310
YAN Zhengyu, SHU Juan, YU Yan, ZHANG Zhengwei, TANG Lu, CHEN Jianqiu. Preparation and application of carbon dots in chloramphenicol determination[J]. Journal of China Pharmaceutical University, 2015, 46(3): 322-327. DOI: 10.11665/j.issn.1000-5048.20150310
Citation: YAN Zhengyu, SHU Juan, YU Yan, ZHANG Zhengwei, TANG Lu, CHEN Jianqiu. Preparation and application of carbon dots in chloramphenicol determination[J]. Journal of China Pharmaceutical University, 2015, 46(3): 322-327. DOI: 10.11665/j.issn.1000-5048.20150310

碳纳米粒的制备及其用于氯霉素的测定

Preparation and application of carbon dots in chloramphenicol determination

  • 摘要: 首次以柠檬酸为碳源、甘氨酸为修饰剂,通过高温热解法一步合成修饰碳纳米粒。经过正丁醇萃取纯化,碳纳米粒的粒径更加均一,荧光强度更大,且性能得到了改善。最终制得的碳纳米粒呈棕黄色,在380 nm激发波长下,最大发射波长出现在480 nm附近,其荧光量子产率高达47%。氯霉素对碳纳米粒的荧光具有显著的猝灭效应,并呈现一定的规律性,据此建立了对氯霉素含量测定的新方法。该方法简便快捷,易于操作,线性关系良好(r=0.999 7),回收率在99%~101%(RSD=0.3%),显示了碳纳米粒在药物检测方面的潜在应用前景。
    Abstract: Carbon dots(CDs)were firstly prepared via a simple pyrolysis route using citric acid as the carbon precursor and glycine as modifier. The obtained CDs were further purified by n-butyl alcohol, which made the particle size more uniform and the fluorescence intensity stronger. When excited at 380 nm, the maximum emission wavelength of the CDs was about 480 nm, with a quantum yield of 47%. Based on fluorescence quenching, a novel method for chloramphenicol assay was developed with glycine-passivated CDs. This method is simple and rapid. A linear relationship between the change of fluorescence intensity and the concentration of chloramphenicol was obtained with a relation coefficient of 0. 999 7. The recovery was in the range of 99% to 101% with a relative standard deviation of 0. 3%, which shows CDs′ potential application in drug detection.
  • [1] Bourlinos AB,Stassinopoulos A,Anglos D,et al.Photoluminescent carbogenic dots[J].Chem Mater,2008,20(14):4539-4541.
    [2] Bourlinos AB,Stassinopoulos A,Anglos D,et al.Surface functionalized carbogenic quantum dots[J].Small,2008,4(4):455-458.
    [3] Peng H,Travas-Sejdic J.Simple aqueous solution route to luminescent carbogenic dots from carbohydrates[J].Chem Mater,2009,21(23):5563-5565.
    [4] Wang YQ,Chen LX.Quantum dots,lighting up the research and development of nanomedicine[J].Nanomedicine: Nano Bio Med,2011,7(4):385-402.
    [5] Zhou JG,Booker C,Li RY,et al.An electrochemical avenue to blue luminescent nanocrystals from multiwalled carbon nanotubes(MWCNTs)[J].J Am Chem Soc,2007,129(4):744-745.
    [6] Xu XY,Ray R,Gu YL,et al.Electrophoretic analysis and purification of fluorescent single-walled carbon nanotube fragments[J].J Am Chem Soc,2004,126(40):12736-12737.
    [7] Wang X,Cao L,Lu FS,et al.Photoinduced electron transfers with carbon dots[J].Chem Commun,2009,(25):3774-3776.
    [8] Ray SC,Saha A,Jana NR,et al.Fluorescent carbon nanoparticles: synthesis,characterization and bioimaging application[J].J Phys Chem C,2009,113(43): 18546-18551.
    [9] Zhou JJ,Sheng ZH,Han HY,et al.Facile synthesis of fluorescent carbon dots using watermelon peel as a carbon source[J].Mater Lett,2012,66(1):222-224.
    [10] Zhu H,Wang XL,Li Y,et al.Microwave synthesis of fluorescent carbon nanoparticles with electrochemiluminescence properties[J].Chem Commun,2009,(34): 5118-5120.
    [11] Liu RL,Wu DQ,Liu SH,et al.An aqueous route to multicolor photoluminescent carbon dots using silica spheres as carriers[J].Angew Chem Int Ed,2009,48(25): 4598-4601.
    [12] Hu SL,Niu KY,Sun J,et al.One-step synthesis of fluorescent carbon nanoparticles by laser irradiation[J].J Mater Chem,2009,19(4):484-488.
    [13] DongYQ,Wang RX,Li H,et al.Polyamine-functionalized carbon quantum dots for chemical sensing[J].Carbon,2012,50(8):2810-2815.
    [14] Sun YP,Wang X,Lu FS,et al.Doped carbon nanoparticles as a new platform for highly photoluminescent dots[J].J Phys Chem C,2008,112(47):18295-18298.
    [15] Cao L,Wang X,Meziani,MJ,et al.Carbon dots for multiphoton bioimaging[J].J Am Chem Soc,2007,129(37):11318-11319.
    [16] Yang ST,Cao L,Luo PG,et al.Carbon dots for optical imaging in vivo[J].J Am Chem Soc,2009,131(32):11308-11309.
    [17] Liu LQ,Li YF.Carbon dots used for the detection of lysozyme with resonance light scattering signals[J].J Southwest Univ(Nat Sci)(东南大学学报:医学版),2010,32(11):25-29.
    [18] Zheng HZ,Bai WJ,Long YJ,et al.A novel carbon dots based fluorescence turn-on method for biothiol determination[J].Sci China Chem(中国科学:化学),2011,41(6):1031-1036.
计量
  • 文章访问数:  1127
  • HTML全文浏览量:  0
  • PDF下载量:  1401
  • 被引次数: 0
出版历程
  • 刊出日期:  2015-06-24

目录

    /

    返回文章
    返回
    x 关闭 永久关闭