• 中国中文核心期刊
  • 中国科学引文数据库核心期刊
  • 中国科技核心期刊
  • 中国高校百佳科技期刊
高级检索

汉黄芩素抑制人胃癌MGC-803细胞糖代谢的作用

尹谦, 戴琴升, 赵亦楷, 郭青龙

尹谦, 戴琴升, 赵亦楷, 郭青龙. 汉黄芩素抑制人胃癌MGC-803细胞糖代谢的作用[J]. 中国药科大学学报, 2015, 46(3): 345-349. DOI: 10.11665/j.issn.1000-5048.20150314
引用本文: 尹谦, 戴琴升, 赵亦楷, 郭青龙. 汉黄芩素抑制人胃癌MGC-803细胞糖代谢的作用[J]. 中国药科大学学报, 2015, 46(3): 345-349. DOI: 10.11665/j.issn.1000-5048.20150314
YIN Qian, DAI Qinsheng, ZHAO Yikai, GUO Qinglong. Inhibitory effects on glucose metabolism of human gastric carcinoma MGC-803 cells induced by wogonin[J]. Journal of China Pharmaceutical University, 2015, 46(3): 345-349. DOI: 10.11665/j.issn.1000-5048.20150314
Citation: YIN Qian, DAI Qinsheng, ZHAO Yikai, GUO Qinglong. Inhibitory effects on glucose metabolism of human gastric carcinoma MGC-803 cells induced by wogonin[J]. Journal of China Pharmaceutical University, 2015, 46(3): 345-349. DOI: 10.11665/j.issn.1000-5048.20150314

汉黄芩素抑制人胃癌MGC-803细胞糖代谢的作用

基金项目: 国家“重大新药创制”科技重大专项资助项目(No.2012ZX09304-001);中央高校基本科研业务费专项资金资助项目(No.PY2014ZY0008)

Inhibitory effects on glucose metabolism of human gastric carcinoma MGC-803 cells induced by wogonin

  • 摘要: 探讨汉黄芩素对人胃癌MGC-803细胞糖代谢的抑制作用及其可能的内在机制。采用葡萄糖摄取检测试剂盒检测汉黄芩素对MGC-803细胞糖代谢中葡萄糖摄取量的影响;同时采用乳酸生成检测试剂盒检测汉黄芩素对MGC-803细胞糖代谢中乳酸生成量的影响;Annexin V-PI双染实验检测汉黄芩素调节糖代谢过程中MGC-803细胞的凋亡率;Western blot法分析糖代谢相关蛋白己糖激酶2(HKⅡ)、葡萄糖转运蛋白1(GLUT1)、丙酮酸脱氢酶激酶(PDHK)和乳酸脱氢酶A(LDH-A)的表达变化情况。结果表明:汉黄芩素能在一定浓度下抑制人胃癌MGC-803细胞糖代谢中的葡萄糖摄取量,同时它还能抑制人胃癌MGC-803细胞糖代谢中的乳酸生成量,但并未诱发明显凋亡。此外一定浓度下的汉黄芩素还能抑制人胃癌MGC-803细胞糖代谢相关蛋白的表达。综上所述,汉黄芩素能抑制人胃癌细胞MGC-803的糖代谢但并未诱发明显凋亡,其抑制作用可能与其对MGC-803细胞糖代谢相关蛋白的作用相关。
    Abstract: To investigate the inhibitory effect of wogonin on glucose metabolism in human gastric carcinoma MGC-803 cells and its underlying mechanism, Amplex Red Glucose/Glucose Oxidase Assay Kit was used to explore the influence of wogonin on glucose uptake of MGC-803 cells and lactate assay kit was adopted to evaluate the lactate generation of MGC-803 cells. Annexin V/PI staining assay was performed to observe the apoptotic rate of MGC-803 cells. Changes in the expression of hexokinase 2(HK II), glucose transporter 1(GLUT1), pyruvate dehydrogenase kinase(PDHK)and lactate dehydrogenase A(LDH-A)were assessed by Western blot. These results demonstrated that wogonin significantly reduced glucose uptake of MGC-803 cells at certain concentration. Meanwhile, wogonin apparently reduced the lactate generation of MGC-803 cells, without inducing notable cell apoptosis. Besides, with the impact of wogonin, the expression of glucose metabolism related proteins declined obviously. In a word, wogonin could inhibit the glucose metabolism of MGC-803 cells without inducing apparent apoptosis, which may be related to its inhibitory influence on the proteins in glucose metabolism of MGC-803 cells.
  • [1] Hamanaka RB, Chandel NS. Targeting glucose metabolism for cancer therapy[J].J Exp Med,2012,209(2):211-215.
    [2] Airley RE,Mobasheri A.Hypoxic regulation of glucose transport,anaerobic metabolism and angiogenesis in cancer:novel pathways and targets for anticancer therapeutics[J].Chemotherapy,2007,53(4):233-256.
    [3] Macheda ML,Rogers S,Best JD.Molecular and cellular regulation of glucose transporter(GLUT)proteins in cancer[J].J Cell Physiol,2005,202(3):654-662.
    [4] Chan DA,Sutphin PD,Nguyen P,et al.Targeting GLUT1 and the Warburg effect in renal cell carcinoma by chemical synthetic lethality[J].Sci Transl Med,2011,3(94):70-94.
    [5] Robey RB,Hay N.Mitochondrial hexokinases,novel mediators of the antiapoptotic effects of growth factors and Akt[J].Oncogene,2006,25(34):4683-4696.
    [6] Jae HJ,Chung JW,Park HS,et al.The antitumor effect and hepatotoxicity of a hexokinase II inhibitor 3-bromopyruvate:in vivo investigation of intraarterial administration in a rabbit VX2 hepatoma model[J].Korean J Radiol,2009,10(6):596-603.
    [7] Kim JW,Gao P,Liu YC,et al.Hypoxia-inducible factor 1 and dysregulated c-Myc cooperatively induce vascular endothelial growth factor and metabolic switches hexokinase 2 and pyruvate dehydrogenase kinase 1[J].Mol Cell Biol,2007,27(21):7381-7393.
    [8] Dang CV,Lewis BC,Dolde C,et al.Oncogenes in tumor metabolism,tumorigenesis,and apoptosis[J].J Bioenerg Biomembr,1997,29(4):345-354.
    [9] Pelicano H, Martin DS, Xu RH, et al. Glycolysis inhibition for anticancer treatment[J].Oncogene,2006,25(34):4633-4646.
    [10] Yang L,You QD,Yan Y,et al.Research advances in wogonin′s anti-tumor effects[J].J China Pharm Univ(中国药科大学学报),2009,40(6):576-579.
    [11] Gao Y,Zhao Y,Yao J,et al.Wogonin suppresses human alveolar adenocarcinoma cell A549 invasion and metastasis induced by IL-6[J].J China Pharm Univ(中国药科大学学报),2014,45(4):456-164.
    [12] Jose C,Bellance N,Rossignol R.Choosing between glycolysis and oxidative phosphorylation:a tumor′s dilemma[J]? Bba-Bioenergetics,2011,1807(6):552-561.
    [13] Zhao K,Wei L,Hui H,et al.Wogonin suppresses melanoma cell B16-F10 invasion and migration by inhibiting Ras-medicated pathways[J].PLoS One,2014,9(9):e106458.
    [14] Song X,Yao J,Wang F,et al.Wogonin inhibits tumor angiogenesis via degradation of HIF-1alpha protein[J].Toxicol Appl Pharmacol,2013,271(2):144-155.
    [15] Yao J,Zhao L,Zhao Q,et al.NF-kappaB and Nrf2 signaling pathways contribute to wogonin-mediated inhibition of inflammation-associated colorectal carcinogenesis[J].Cell Death Dis,2014,5:e1283.
    [16] Wang H,Zhao L,Zhu LT,et al.Wogonin reverses hypoxia resistance of human colon cancer HCT116 cells via downregulation of HIF-1alpha and glycolysis,by inhibiting PI3K/Akt signaling pathway[J].Mol Carcinog,2014,53(Suppl 1):E107-118.
    [17] Yang H,Hui H,Wang Q,et al.Wogonin induces cell cycle arrest and erythroid differentiation in imatinib-resistant K562 cells and primary CML cells[J].Oncotarget,2014,5(18):8188-8201.
计量
  • 文章访问数:  1569
  • HTML全文浏览量:  0
  • PDF下载量:  1519
  • 被引次数: 0
出版历程
  • 刊出日期:  2015-06-24

目录

    /

    返回文章
    返回
    x 关闭 永久关闭