高级检索

含聚多价适配体的长链DNA用于抗肿瘤药物的靶向传输

A DNA polyaptamer system as a targeted antitumor drug delivery

  • 摘要: 采用滚环扩增(RCA)方法合成含聚多价适配体的长链DNA(polyaptamer),并负载抗肿瘤药物多柔比星(Dox),用于靶向治疗白血病肿瘤细胞。研究结果表明,含聚多价适配体的polyaptamer载药能力比单价适配体(monoaptamer)提高了10倍以上,而其对急性淋巴白血病细胞(CCRF-CEM)的靶向效率比单价适配体提高了约35倍,极大降低了Dox对正常体细胞的不良反应。药物释放实验和肿瘤细胞杀灭实验同时证明,在酶的作用下,polyaptamer/Dox复合物进入肿瘤细胞后能够短时间内释放出Dox,从而高效地杀灭肿瘤细胞。

     

    Abstract: A long chain structure of DNA(polyaptamer)composed of multiple aptamer units was synthesized by rolling circle amplification and used for the construction of polyaptamer-doxorubicin system in the treatment of leukemia cells. It was found that the system was significantly more effective than monoaptamer in targeting and killing leukemia cells as it provided 35-fold enhanced binding affinity and 10-fold greater drug loading via multivalent effects. Drug release and cell viability also proved that the conjugates could gain entrance into the cells and rapidly release doxorubicin under the action of lysosome, leading to the tumoricidal effect.

     

/

返回文章
返回