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一种快速检测中药材中二氧化硫残留量的荧光探针的合成及其应用

周琳, 刘巍, 狄斌, 陈金龙

周琳, 刘巍, 狄斌, 陈金龙. 一种快速检测中药材中二氧化硫残留量的荧光探针的合成及其应用[J]. 中国药科大学学报, 2015, 46(4): 444-449. DOI: 10.11665/j.issn.1000-5048.20150410
引用本文: 周琳, 刘巍, 狄斌, 陈金龙. 一种快速检测中药材中二氧化硫残留量的荧光探针的合成及其应用[J]. 中国药科大学学报, 2015, 46(4): 444-449. DOI: 10.11665/j.issn.1000-5048.20150410
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ZHOU Lin, LIU Wei, DI Bin, CHEN Jinlong. Synthesis and application of a fluorescent molecular probe for rapid detection of sulfur dioxide residues in traditional Chinese herbs[J]. Journal of China Pharmaceutical University, 2015, 46(4): 444-449. DOI: 10.11665/j.issn.1000-5048.20150410
Citation: ZHOU Lin, LIU Wei, DI Bin, CHEN Jinlong. Synthesis and application of a fluorescent molecular probe for rapid detection of sulfur dioxide residues in traditional Chinese herbs[J]. Journal of China Pharmaceutical University, 2015, 46(4): 444-449. DOI: 10.11665/j.issn.1000-5048.20150410
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一种快速检测中药材中二氧化硫残留量的荧光探针的合成及其应用

  • 1.

    中国药科大学药物分析教研室

  • 2.

    南京师范大学能源与机械工程学院

  • 3.

    中国药科大学药物质量与安全预警教育部重点实验室

基金项目: 国家自然科学基金资助项目(No.21305161)；江苏省自然科学基金资助项目(No.BK20130643)；中央高校基本科研业务费专项资金资助项目(No.ZJ13068)

Synthesis and application of a fluorescent molecular probe for rapid detection of sulfur dioxide residues in traditional Chinese herbs

摘要

    摘要
  • 摘要: 建立一种增强型(turn-on)罗丹明衍生物荧光探针检测HSO-3和硫磺熏蒸中药材中二氧化硫残留量的方法。此方法基于探针中的醛基和HSO-3在pH 4.8的水醇条件下发生加成反应，伴随有罗丹明衍生物中的螺内酰胺开环可使探针荧光明显增强，荧光增强程度与HSO-3的浓度呈线性相关，线性范围0.005~20 μmol/L，相关系数r=0.998 5。此方法对HSO-3具有高灵敏度和选择性，检测限低至2.0 nmol/L。常见的阴阳离子及还原性物质对HSO-3的检测均没有干扰。此外，将此荧光探针用于检测硫磺熏蒸中药材二氧化硫残留量，操作简便、快速，回收率良好。
    Abstract: A fluorescence method based on a “turn-on” rhodamine derivative fluorescent probe was developed for the detection of hydrogen sulfite ions and sulfur dioxide residues in sulfur-fumigated herbs. This method was realized through a well-known aldehyde-bisulfite addition reaction accompanied by a ring opening of spirolactam of probe molecule in pH 4. 8 aqueous ethanol media which resulted in a significant fluorescence and color change. The fluorescence enhancement was linearly proportional to the concentrations of bisulfite ranging from 0. 005 to 20 μmol/L with a correlation coefficient of 0. 998 5. The detection limit was low as to 2. 0 nmol/L and there was no interference with other familiar co-existing anions, cations and reducing reagents indicating high sensitivity and selectivity of the proposed method. In addition, the novel probe was successfully applied for the identification of sulfur dioxide residues in herbs with a satisfactory recovery in five real samples.
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    • 参考文献(14)
  • [1] Wang XH,Xie PS,Lam CW,et al.Study of the destructive effect to inherent quality of Angelicae dahuricae radix by sufur-fumigated process using chromatographic fingerprinting analysis[J].J Pharm Biomed Anal,2009,49(5):1221-1225.
    [2] Kan WL,Ma B,Lin G.Sulfur fumigation processing of traditional Chinese medicinal herbs:benefical or detrimental[J].Front Pharmacol,2011,2(1):1-7.
    [3] Yang XF, Guo XQ, Zhao YB, et al. Novel spectrofluorimetric method for the determination of sulfite with rhodamine B hydrazide in a micellar medium[J].Anal Chim Acta,2002,456(1):121-128.
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    [5] Chinese Pharmacopoeia Commission.Chinese Pharmcopoeia:second supplements,Part 1(中华人民共和国药典:第二增补本)[M].Beijing:China Medical Science Press,2013:180.
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    [8] Sakchai S, Pornthana P, Saisunee L. Preparition flow injection analysis for the determination of sulphite in food samples utilising potassium permanganate-rhodamine B chemiluminesce detection[J].Food Chem,2010,121(3):893-898.
    [9] Miura Y,Fujisaki M,Haddad PR.Spectrophotometric determination of sulfide in the presence of sulfite and thiosulfate via the precipitation of bismuth(III)sulfide[J].Anal Sci,2004,20(2):363-367.
    [10] Malakootian M,Beitollah H,Biparva P.Nanostructured base electrochemical sensor for determination of sulfite[J].Int J Electrochem Sc,2014,9(1):327-341.
    [11] de Macedo AN,Macri J,Belostotsky V,et al.Strong anion determination in biological fluids by capillary electrophoresis for clinical diagnostics[J].Anal Chem,2013,85(22):11112-11120.
    [12] Huang T,OU-yang H,Shi Z,et al.Progress on metal ions fluorescent probe based on the off-on mechanism of rhodamine derivatives[J].J Xiamen Univ(厦门大学学报),2011,50(9):137-140.
    [13] Duong TQ,Kim JS.Fluoro- and chromogenic chemodosimeters for heavy metal ion detection in solution and biospecimens[J].Chem Rev,2010,110(10):6280-6301.
    [14] Chinese Pharmacopoeia Commission.Chinese Pharmcopoeia:Part 1(中华人民共和国药典:一部)[M].Beijing:China Medical Science Press,2013:180.
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  • 刊出日期:  2015-08-24

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