黄酮类化合物GL-V9对人胃低分化黏液腺癌细胞株的凋亡作用及其机制
Effects and mechanism of programmed cell death of human gastric cancer cell under the treatment of flavonoid compound GL-V9
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摘要: 探讨黄酮类化合物GL-V9对人体低分化胃黏液腺癌细胞系MGC-803和BGC-823的凋亡作用及其机制。采用MTT法观察不同浓度的GL-V9作用于两种胃癌细胞后对其细胞活力的影响。采用Annexin V-FITC/PI双染法、DAPI染色法观察GL-V9对MGC-803细胞株凋亡率和细胞核形态的影响。采用免疫印迹法观察GL-V9对MGC-803细胞株中主要凋亡蛋白caspase-9，caspase-3的影响。应用钙离子荧光探针Fluo-3 AM检测GL-V9对MGC-803细胞株内钙离子浓度变化的影响。实验结果表明，GL-V9可以抑制胃癌细胞株MGC-803，BGC-823细胞活性，改变MGC-803细胞核形态，激活caspase-9，caspase-3蛋白，诱导细胞凋亡。同时，GL-V9可以显著上调MGC-803细胞中钙离子水平，这可能与GL-V9作用下的胃癌细胞株中线粒体凋亡通路的激活相关。Abstract: To investigate the apoptotic effect of flavonoid compound GL-V9 on human gastric cancer cells and its potential mechanism, MGC-803 and BGC-823 cells were treated with GL-V9. MTT assay was performed to assess the growth inhibition effects on MGC-803 and BGC-823 cells under different concentrations of GL-V9. Annexin V-FITC/PI staining assay was employed to observe the apoptotic rate of GL-V9 cells with the treatment of GL-V9. DAPI staining was performed to observe the nuclear morphological changes using fluorescence microscopy. Activation of caspase-9 and caspase-3 was analyzed by Western blotting. Ca2+ concentration in gastric cancer cells was detected by Fluo-3 AM staining assay. Results showed that GL-V9 could inhibitcell viability, change the nuclear morphologyl, activate caspase-9 and caspase-3 and induce the apoptosis in gastric cancer cells. The mechanism of the induction of apoptosis in MGC-803 cells under the treatment of GL-V9 may aetivate the Ca2+ associated mitochondrial apoptosis pathway.
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Keywords:
- flavonoid compound /
- GL-V9 /
- gastric adenocarcinoma /
- MGC-803 cell /
- apoptosis /
- Ca2+
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