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单细胞蛋白定量检测方法研究进展

沈燚昀, 齐谢敏, 宋沁馨, 周国华

沈燚昀, 齐谢敏, 宋沁馨, 周国华. 单细胞蛋白定量检测方法研究进展[J]. 中国药科大学学报, 2015, 46(5): 521-531. DOI: 10.11665/j.issn.1000-5048.20150502
引用本文: 沈燚昀, 齐谢敏, 宋沁馨, 周国华. 单细胞蛋白定量检测方法研究进展[J]. 中国药科大学学报, 2015, 46(5): 521-531. DOI: 10.11665/j.issn.1000-5048.20150502
shu
SHEN Yiyun, QI Xiemin, SONG Qinxin, ZHOU Guohua. Research advances in quantitative detection of single cell protein[J]. Journal of China Pharmaceutical University, 2015, 46(5): 521-531. DOI: 10.11665/j.issn.1000-5048.20150502
Citation: SHEN Yiyun, QI Xiemin, SONG Qinxin, ZHOU Guohua. Research advances in quantitative detection of single cell protein[J]. Journal of China Pharmaceutical University, 2015, 46(5): 521-531. DOI: 10.11665/j.issn.1000-5048.20150502
shu

单细胞蛋白定量检测方法研究进展

  • 1.

    药物质量与安全预警教育部重点实验室(中国药科大学)

  • 2.

    中国药科大学药物分析教研室

  • 3.

    南京军区南京总医院药理科

基金项目: 江苏省科技支撑计划社会发展项目资助项目(No.BE2012744)；江苏省自然科学基金资助项目(No.BK20151445)；中国博士后科学基金资助项目(No.2012M512179,No.2013T60962)；中央高校基本科研业务费重点资助项目(No.2015ZD008)；药物质量与安全预警教育部重点实验室资助项目(No.DQCP2015MS02)；江苏高校优势学科建设工程资助项目；江苏省高校“青蓝工程”资助项目

Research advances in quantitative detection of single cell protein

摘要

    摘要
  • 摘要: 蛋白质的存在及表达水平差异可以阐明生物体的生理或病理变化机制，因此蛋白质的定量检测对疾病发生机制研究、疾病诊断和预后评价等具有重要意义。传统的组织水平上的蛋白定量检测反映的是细胞中蛋白质表达量的平均水平，它往往忽略了单个细胞之间存在的差异，而单细胞蛋白定量检测手段则更能真实地反映这种差异。近年来发展了一系列单细胞蛋白定量检测的方法，如微流控技术、微孔技术、光导纤维纳米生物传感器技术、基于活性的荧光探针技术和质谱法等，本文就这些方法的原理、优缺点等进行简要介绍。
    Abstract: Proteins presence and differences of the expression level can clarify the physiological or pathological changes in organisms, so the quantitative detection of proteins is vital for disease mechanism research, diagnosis and prognosis evaluation. Traditional protein quantitation methods at the tissue level reflected the average protein expression in cells, but ignore the differences between individual cells. In contrast, approaches for quantitative detection at single-cell level can better reflect the differences. Recently, a number of approaches for such detection have been proposed, including microfluidics, microwell-based technology, optical fiber nanobiosensor, activity-based probe technology and mass spectrometry. The principles, advantages and drawbacks of these approaches are briefly introduced in this review.
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  • 刊出日期:  2015-10-24

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