• 中国中文核心期刊
  • 中国科学引文数据库核心期刊
  • 中国科技核心期刊
  • 中国高校百佳科技期刊
高级检索

栀子大黄汤对酒精性肝损伤保护作用的质谱评价

李欣昕, 陈林飞, 欧阳永中, 陈焕文, 冯芳

李欣昕, 陈林飞, 欧阳永中, 陈焕文, 冯芳. 栀子大黄汤对酒精性肝损伤保护作用的质谱评价[J]. 中国药科大学学报, 2015, 46(5): 579-586. DOI: 10.11665/j.issn.1000-5048.20150511
引用本文: 李欣昕, 陈林飞, 欧阳永中, 陈焕文, 冯芳. 栀子大黄汤对酒精性肝损伤保护作用的质谱评价[J]. 中国药科大学学报, 2015, 46(5): 579-586. DOI: 10.11665/j.issn.1000-5048.20150511
shu
LI Xinxin, CHEN Linfei, OUYANG Yongzhong, CHEN Huanwen, FENG Fang. Mass spectrometry assessment of the protective effects of Zhizi Dahuang decoction on alcoholic liver injury[J]. Journal of China Pharmaceutical University, 2015, 46(5): 579-586. DOI: 10.11665/j.issn.1000-5048.20150511
Citation: LI Xinxin, CHEN Linfei, OUYANG Yongzhong, CHEN Huanwen, FENG Fang. Mass spectrometry assessment of the protective effects of Zhizi Dahuang decoction on alcoholic liver injury[J]. Journal of China Pharmaceutical University, 2015, 46(5): 579-586. DOI: 10.11665/j.issn.1000-5048.20150511
shu

栀子大黄汤对酒精性肝损伤保护作用的质谱评价

  • 1.

    中国药科大学药物分析教研室

  • 2.

    东华理工大学江西省质谱科学与仪器重点实验室

基金项目: 国家自然科学基金资助项目(No.21225522,No.81274063,No.21265001)

Mass spectrometry assessment of the protective effects of Zhizi Dahuang decoction on alcoholic liver injury

摘要

    摘要
  • 摘要: 采用液体辅助表面解吸常压化学电离质谱(LA-DAPCI-MS)技术对正常大鼠肝脏(正常组)、急性酒精性肝损伤大鼠肝脏(模型组)和接受栀子大黄汤治疗的肝损伤大鼠肝脏(治疗组)3组切片进行质谱成像研究，结合主成分分析(PCA)法对3组大鼠肝脏进行区分，识别出不同组动物肝内的差异性磷酸胆碱PC(34∶2)，PC(36∶4)和PC(38∶4)。成像结果显示，在0.01 mm2的空间分辨率下，3种磷酸胆碱在肝组织中分布较均匀，在模型组中的含量相较于正常组和治疗组均降低，揭示了急性酒精性肝损伤与肝内磷脂变化的关系，在分子水平上评价了栀子大黄汤对急性酒精性肝损伤引起的肝内磷脂变化的保护作用。
    Abstract: Liquid-assisted surface desorption atmospheric pressure chemical ionization mass spectrometry(LA-DAPCI-MS)was used directly for the analysis of healthy rat liver(normal control group), acute alcoholic injury rat liver(alcohol model group)and Zhizi Dahuang decoction-treated rat liver(ZZDHD group)sections with the mass spectrometry images obtained simultaneously. Principal component analysis(PCA)was employed for the differentiation of livers of the groups, and 3 phospholipids, PC(34 ∶2), PC(36 ∶4)and PC(38 ∶4), were found to be the main factors. LA-DAPCI mass spectrometry images showed that the 3 phospholipids were evenly distributed in liver under the spatial resolution of 0. 01 mm2. Contents of 3 PCs in the model group were decreased in alcohol model group compared to the normal control group and the ZZDHD group, which revealed the relationship between acute alcoholic liver injury and intrahepatic phospholipid variation. The results showed that ZZDHD had protective effect on acute alcoholic liver injury-induced intrahepatic phospholipid variations at the molecular level.
    • HTML全文
    • 参考文献(24)
  • [1] Orman ES,Odena G,Bataller R.Alcoholic liver disease:pathogenesis,management,and novel targets for therapy[J].J Gastroenterol Hepatol,2013,28(S1):77-84.
    [2] Wang H.Preliminary studies on the therapeutic material basis of Zhizi Dahuang decoction on the hepatoprotective effect against acute alcohol-induced liver injury(栀子大黄汤对急性酒精性肝损伤保护作用的药效物质基础初步研究)[D].Nanjing:China Pharmaceutical University,2009.
    [3] Ding RB,Tian K,Cao YW,et al.Protective effect of Panax notoginseng Saponins on acute ethanol-induced liver injury is associated with ameliorating hepatic lipid accumulation and reducing ethanol-mediated oxidative stress[J].J Agric Food Chem,2015,63(9):2413-2422.
    [4] Zhang P,Ma D,Wang Y,et al.Berberine protects liver from ethanol-induced oxidative stress and steatosis in mice[J].Food Chem Toxicol,2014,74:225-232.
    [5] An L,Feng F.Network pharmacology-based antioxidant effect study of Zhi-Zi-Da-Huang decoction for alcoholic liver disease[J].Evid Based Complement Alternat Med,2015,2015:492470.
    [6] Zhu H,Bi K,Han F,et al.Identification of the absorbed components and metabolites of Zhi-Zi-Da-Huang decoction in rat plasma by ultra-high performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry[J].J Pharm Biomed Anal,2015,111:277-287.
    [7] Guo S,Wang Y,Zhou D,et al.Significantly increased monounsaturated lipids relative to polyunsaturated lipids in six types of cancer microenvironment are observed by mass spectrometry imaging[J].Sci Rep,2014,4:5959.
    [8] Debois D,Bralet MP,Le Naour F,et al.In situ lipidomic analysis of nonalcoholic fatty liver by cluster TOF-SIMS imaging[J].Anal Chem,2009,81(8):2823-2831.
    [9] Abd El-Kader SM,El-Den Ashmawy EM.Non-alcoholic fatty liver disease:the diagnosis and management[J].World J Hepatol,2015,7(6):846-858.
    [10] Wattacheril J,Seeley EH,Angel P,et al.Differential intrahepatic phospholipid zonation in simple steatosis and nonalcoholic steatohepatitis[J].PLoS One,2013,8(2):e57165.
    [11] Moustafa T,Fickert P,Magnes C,et al.Alterations in lipid metabolism mediate inflammation,fibrosis,and proliferation in a mouse model of chronic cholestatic liver injury[J].Gastroenterology,2012,142(1):140-151.
    [12] Park ES,Lee JH,Hong JH,et al.Phosphatidylcholine alteration identified using MALDI imaging MS in HBV-infected mouse livers and virus-mediated regeneration defects[J].PLoS One,2014,9(8):e103955.
    [13] Li XX,Wu H,Wang C,et al.Mass spectrometry imaging and its application in pharmaceutical science[J].J China Pharm Univ(中国药科大学学报),2014,45(1):17-25.
    [14] Jia B.Surface desorption atmospheric pressure chemical ionization:mechanism and the applications in the analysis of food and pharmaceutical samples(表面解吸常压化学电离的机理及其在食品和医药样品分析中的应用研究)[D].Nanchang:East China Institute of Technology,2012.
    [15] Jiang CC,Luo LP,Hu B,et al.In situ detection of sinapine and its decline studied in radish taproot by surface desorption atmospheric pressure chemical ionization mass spectrometry[J].Chin J Appl Chem(应用化学),2011,28(4):432-437.
    [16] Wang H,Feng F,Zhuang B Y,et al.Evaluation of hepatoprotective effect of Zhi-Zi-Da-Huang decoction and its two fractions against acute alcohol-induced liver injury in rats[J].J Ethnopharmacol,2009,126(2):273-279.
    [17] Zou LL,Li XX,Shi QS,et al.An effective integrated method for comprehensive identification of eighty-five compounds in Zhi-Zi-Da-Huang decoction by HPLC-DAD-ESI-MS(TOF)and HPLC-DAD-ESI-MS/MS(QqQ)without the help of reference standards[J].Anal Methods,2014,6:4312-4327.
    [18] Yao AM,Huang JQ,Xu DX,et al.Quantitation of multiple constituents in Zhizi Dahuang decoction by RP-HPLC and its application in the study of chemical components in herb compatibility[J].J China Pharm Univ(中国药科大学学报),2013,44(6):531-535.
    [19] Wiseman JM,Puolitaival SM,Takáts Z,et al.Mass spectrometric profiling of intact biological tissue by using desorption electrospray ionization[J].Angew Chem Int Ed Engl,2005,44(43):7094-7097.
    [20] Goto-Inoue N,Hayasaka T,Zaima N,et al.Imaging mass spectrometry for lipidomics[J].Biochim Biophys Acta,2011,1811(11):961-969.
    [21] Manicke NE,Dill AL,Ifa DR,et al.High-resolution tissue imaging on an orbitrap mass spectrometer by desorption electrospray ionization mass spectrometry[J].J Mass Spectrom,2010,45(2):223-226.
    [22] Manicke NE,Nefliu M,Wu C,et al.Imaging of lipids in atheroma by desorption electrospray ionization mass spectrometry[J].Anal Chem,2009,81(21):8702-8707.
    [23] Cooks RG,Manicke NE,Dill AL,et al.New ionization methods and miniature mass spectrometers for biomedicine:DESI imaging for cancer diagnostics and paper spray ionization for therapeutic drug monitoring[J].Faraday Discuss,2011,149(1):247-267.
    [24] Eberlin LS,Ferreira CR,Dill AL,et al.Desorption electrospray ionization mass spectrometry for lipid characterization and biological tissue imaging[J].Biochim Biophys Acta,2011,1811(11):946-960.
    • 相关文章
    • 施引文献
    • 资源附件(0)
计量
  • 文章访问数:  1224
  • HTML全文浏览量:  1
  • PDF下载量:  2114
  • 被引次数: 0
出版历程
  • 刊出日期:  2015-10-24

目录

摘要
HTML全文
    参考文献(24)
    相关文章
    施引文献
    资源附件(0)

    /

    返回文章
    返回

    版权所有：《中国药科大学学报》编辑部苏ICP备05007142号

    地址：江苏省南京市鼓楼区童家巷24号（210009）电话: 025-83271566E-mail: xuebao@cpu.edu.cn

    本系统由 北京仁和汇智信息技术有限公司 开发  百度统计

    访问量：309662

    x 关闭 永久关闭