Abstract: This study aimed to detect the combined effects of gallic acid(GA)and ciprofloxacin(CIP)on the murine chronic rhinosinusitis(CRS)model in mice. Pseudomonas aeruginosa from refractory CRS nasal samples were isolated and a CRS model in mice was induced. GA and CIP were intragastrically administered singly or in combination. The nasal histopathologic change was observed by hematoxylin and eosin(HE)staining. The concentration of TNF-α, IL-6 and IL-8 in serum were determined by ELISA assay. The activity of SOD and contents of MDA and ROS were measured with commercially available kits. The expressions of IκB, NF-κB p65, TNF-α, IL-6 and IL-8 in nasal mucosa tissues were measured by Western blotting assay. The results showed that the inflammation of CRS in each treatment group was significantly attenuated. The expression level of TNF-α, IL-6, IL-8, MDA, NF-κB p65 and the contents of ROS were reduced significantly in treated groups, while the activity of SOD and the expression level of IκB were increased. More obvious effects were achieved in CA and CIP combined group. The data showed that combination of GA and CIP was superior to GA or CIP alone, and the combined therapy might be related with inhibiting NF-κB signaling pathway and downregulating the expressions of TNF-α, IL-6 and IL-8.