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没食子酸与环丙沙星联用对小鼠慢性鼻-鼻窦炎模型的作用

姜玉秋, 唐桥斐, 张爽, 闫智永

姜玉秋, 唐桥斐, 张爽, 闫智永. 没食子酸与环丙沙星联用对小鼠慢性鼻-鼻窦炎模型的作用[J]. 中国药科大学学报, 2015, 46(5): 600-604. DOI: 10.11665/j.issn.1000-5048.20150514
引用本文: 姜玉秋, 唐桥斐, 张爽, 闫智永. 没食子酸与环丙沙星联用对小鼠慢性鼻-鼻窦炎模型的作用[J]. 中国药科大学学报, 2015, 46(5): 600-604. DOI: 10.11665/j.issn.1000-5048.20150514
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JIANG Yuqiu, TANG Qiaofei, ZHANG Shuang, YAN Zhiyong. Combined effects of gallic acid and ciprofloxacin on the murine chronic rhinosinusitis model in mice[J]. Journal of China Pharmaceutical University, 2015, 46(5): 600-604. DOI: 10.11665/j.issn.1000-5048.20150514
Citation: JIANG Yuqiu, TANG Qiaofei, ZHANG Shuang, YAN Zhiyong. Combined effects of gallic acid and ciprofloxacin on the murine chronic rhinosinusitis model in mice[J]. Journal of China Pharmaceutical University, 2015, 46(5): 600-604. DOI: 10.11665/j.issn.1000-5048.20150514
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没食子酸与环丙沙星联用对小鼠慢性鼻-鼻窦炎模型的作用

  • 沈阳医学院附属第二医院耳鼻喉科

基金项目: 辽宁省科学技术计划项目(No.2012225019)

Combined effects of gallic acid and ciprofloxacin on the murine chronic rhinosinusitis model in mice

摘要

    摘要
  • 摘要: 探讨没食子酸与环丙沙星联用对小鼠慢性鼻-鼻窦炎模型(CRS)的作用。收集难治性CRS患者鼻黏膜样本，分离铜绿假单胞杆菌(Pseudomonas aeruginosa)构建小鼠CRS模型，没食子酸(GA)与环丙沙星(CIP)单独或联合灌胃治疗，HE染色观察小鼠鼻黏膜病理变化，ELISA检测血清炎性因子TNF-α、IL-6、IL-8的表达、试剂盒检测SOD活性、MDA及ROS含量，Western blot检测鼻黏膜组织中TNF-α、IL-6、IL-8、IκB及NF-κB p65的表达。结果显示，GA与CIP可显著降低CRS小鼠TNF-α、IL-6、IL-8、MDA、ROS及NF-κB p65的含量，增加SOD活性及IκB的表达，且联合用药效果更显著。结果说明，GA与CIP联用治疗CRS效果优于单独用药，可能通过抑制NF-κB信号通路，下调TNF-α、IL-6、IL-8表达发挥作用。
    Abstract: This study aimed to detect the combined effects of gallic acid(GA)and ciprofloxacin(CIP)on the murine chronic rhinosinusitis(CRS)model in mice. Pseudomonas aeruginosa from refractory CRS nasal samples were isolated and a CRS model in mice was induced. GA and CIP were intragastrically administered singly or in combination. The nasal histopathologic change was observed by hematoxylin and eosin(HE)staining. The concentration of TNF-α, IL-6 and IL-8 in serum were determined by ELISA assay. The activity of SOD and contents of MDA and ROS were measured with commercially available kits. The expressions of IκB, NF-κB p65, TNF-α, IL-6 and IL-8 in nasal mucosa tissues were measured by Western blotting assay. The results showed that the inflammation of CRS in each treatment group was significantly attenuated. The expression level of TNF-α, IL-6, IL-8, MDA, NF-κB p65 and the contents of ROS were reduced significantly in treated groups, while the activity of SOD and the expression level of IκB were increased. More obvious effects were achieved in CA and CIP combined group. The data showed that combination of GA and CIP was superior to GA or CIP alone, and the combined therapy might be related with inhibiting NF-κB signaling pathway and downregulating the expressions of TNF-α, IL-6 and IL-8.
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  • 刊出日期:  2015-10-24

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