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重组凝血因子Ⅶ表达和合成机制的研究进展

Research advances of recombinant coagulation factor VII expression and synthesizing mechanism

  • 摘要: 血友病是由于人体内缺乏相应凝血因子造成的凝血障碍,目前主要利用基因重组技术在不同细胞中表达重组凝血因子Ⅶ(Ⅶ因子)从而对血友患者出血进行治疗。为有效提高重组Ⅶ因子的产量及活性,已有研究者尝试利用不同真核表达系统合成重组Ⅶ因子,如BHK细胞、CHO细胞、昆虫细胞和鱼胚胎等。同时,不同外源功能性基因对重组Ⅶ因子活性和翻译后修饰对表达产量的影响也通过不同方法进行探究。本文从Ⅶ因子在凝血途径中的作用、重组表达重组Ⅶ因子和翻译后修饰对合成的影响3个方面对重组Ⅶ因子表达和合成机制研究进行了综述。

     

    Abstract: Haemophilia is caused by lack of coagulation factor VIII or IX in patients′ blood with inadequate hemostasis. Currently recombinant coagulation factor VII(rFVII)produced in different cells is used against clinical bleeding of haemophilia patients. To enhance the production and activity of rFVII, some eukaryotic cells such as baby hamster kidney(BHK), Chinese hamster ovary(CHO), insect cell and fish embryo, were used to express rFVII. Meanwhile, the effect of functional gene on the activity of rFVII and the limitation of rFVII production caused by post-translational modification were investigated by different methods. The role of rFVII in hemostasis, synthesis of rFVII in different eukaryotic cells and impact on production of post-translational modification are reviewed in this article.

     

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