• 中国中文核心期刊
  • 中国科学引文数据库核心期刊
  • 中国科技核心期刊
  • 中国高校百佳科技期刊
高级检索

短小舌根草苷的制备及其在大鼠体内代谢产物的UPLC-QTOF/MS鉴定

陈家全, 王慧, 张志远, 翟小婷, 殷蓉, 朱粉霞

陈家全, 王慧, 张志远, 翟小婷, 殷蓉, 朱粉霞. 短小舌根草苷的制备及其在大鼠体内代谢产物的UPLC-QTOF/MS鉴定[J]. 中国药科大学学报, 2015, 46(6): 677-682. DOI: 10.11665/j.issn.1000-5048.20150607
引用本文: 陈家全, 王慧, 张志远, 翟小婷, 殷蓉, 朱粉霞. 短小舌根草苷的制备及其在大鼠体内代谢产物的UPLC-QTOF/MS鉴定[J]. 中国药科大学学报, 2015, 46(6): 677-682. DOI: 10.11665/j.issn.1000-5048.20150607
CHEN Jiaquan, WANG Hui, ZHANG Zhiyuan, ZHAI Xiaoting, YIN Rong, ZHU Fenxia. Preparation of pumiloside and identification of its metabolites in rats by UPLC-QTOF/MS[J]. Journal of China Pharmaceutical University, 2015, 46(6): 677-682. DOI: 10.11665/j.issn.1000-5048.20150607
Citation: CHEN Jiaquan, WANG Hui, ZHANG Zhiyuan, ZHAI Xiaoting, YIN Rong, ZHU Fenxia. Preparation of pumiloside and identification of its metabolites in rats by UPLC-QTOF/MS[J]. Journal of China Pharmaceutical University, 2015, 46(6): 677-682. DOI: 10.11665/j.issn.1000-5048.20150607

短小舌根草苷的制备及其在大鼠体内代谢产物的UPLC-QTOF/MS鉴定

基金项目: 国家自然科学基金资助项目(No.81203001,No.81160558)

Preparation of pumiloside and identification of its metabolites in rats by UPLC-QTOF/MS

  • 摘要: 通过AB-8大孔吸附树脂对胆木药材水提液进行吸附,用不同比例的乙醇水溶液对其梯度洗脱,通过分离纯化获得纯度较高的短小舌根草苷单体。用UPLC-QTOF/MS分析技术鉴定大鼠灌胃短小舌根草苷后,尿液、粪便以及胆汁中该成分可能的代谢产物。采用全扫描方式采集所有成分准确MS/MS数据,利用MetaboLynx软件分析处理,获得了纯度较高的短小舌根草苷单体并鉴定出了其在大鼠体内的4种代谢产物。
    Abstract: In this paper, water extract of Nauclea officinalis was absorbed by AB-8 macroporous resin and subsequently gradient-eluted with alcoholic solution of different proportion to prepare pumiloside monomer of high purity. The metabolites in urine, feces and bile of rats with gavage administration were analyzed by UPLC-QTOF/MS. Accurate MS/MS data of all components were collected with full scan mode, and analyzed by MetaboLynx software. Results showed that the monomer of high-purity pumiloside was prepared and four metabolites in rats were identified.
  • [1] Hu X.Nauclea officinalis:Chemical components and pharmacological effects(乌檀化学成分分离分析与相关成分活性、药动学研究)[D].Shenyang:Shenyang Pharmaceutical University,2007.
    [2] Zhu FX,Wang JJ,Song J,et al.Chemical constituents of Nauclea officinalis[J].Acta Pharm Sin(药学学报),2013,48(2):276-280.
    [3] Chen JQ,Xu GF,Wang H,et al.Simultaneous determination of six main compounds in Nauclea officinalis by ultra-perfoumance liquid chromatography[J].J China Pharm Univ(中国药科大学学报),2014,45(4):434-437.
    [4] Du YX,Yin R,Zhu FX,et al.Analysis of the constituents absorbed into rat blood after intramuscular administration of Danmu injection by UPLC-PDA-QTOF/MS[J].Chin Tradit Pat Med(中成药),2014,36(11):2339-2344.
    [5] Meng Z,Ding G,Liu Y,et al.Study on purification of strictosamide from Nauclea officinalis by macroporous resin[J].Chin J Chin Mater Med(中国中药杂志),2011,36(8):1007-1010.
    [6] Kitajima M.Chemical studies on monoterpenoid indole alkaloids from medicinal plant resources Gelsemium and Ophiorrhiza[J].J Nat Med,2007,61(1):14-23.
    [7] Xuan WD,Bian J,Chen HS.Studies on the separation and purification of pumiloside from Nauclea officinalis by macroporous resin[J].J Chin Med Mater(中药材),2007,30(10):1301-1304.
    [8] Wang JJ.The chemical constituents and material basis investigation of Nauclea offcinalis on anti-inflammatory effects(胆木注射液化学成分及其抗炎物质基础研究)[D].Zhenjiang:Jiangsu University,2012.
    [9] Zhu FX,Chen JQ,Wang JJ,et al.Qualitative and quantitative analysis of the constituents in Danmu preparations by UPLC-PDA-TOF-MS[J].J Chromatogr Sci,2014,52(8):862-871.
    [10] Lu LL,Shu Y,Qian DW,et al.Identification of the metabolites of Sinisan extract in rat plasma,urine,feces and bile after intragastric administration[J].Acta Pharm Sin(药学学报),2011,46(11):1374-1379.
    [11] Zhang H,Zhang D,Ray K,et al.Mass defect filter technique and its applications to drug metabolite identification by high-resolution mass spectrometry[J].J Mass Spectrom,2009,44:999-1016.
计量
  • 文章访问数:  1324
  • HTML全文浏览量:  2
  • PDF下载量:  1563
  • 被引次数: 0
出版历程
  • 刊出日期:  2015-12-24

目录

    /

    返回文章
    返回
    x 关闭 永久关闭