杏芎氯化钠注射液对大鼠脑缺血再灌注损伤的保护作用及机制

任弋; 刘鄂湖; 兰丽; 宋洋洋; 徐阳美; 季晖

doi:10.11665/j.issn.1000-5048.20150614

杏芎氯化钠注射液对大鼠脑缺血再灌注损伤的保护作用及机制

1.
中国药科大学药理学教研室
2.
天然药物活性组分与药效国家重点实验室

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- 刊出日期: 2015-12-24

Protective effects and possible mechanism of Xingxiong sodium chloride injection on cerebral ischemia-reperfusion injury in rats

摘要

摘要: 研究杏芎氯化钠注射液对大鼠局灶性脑缺血再灌注损伤的影响。将大鼠随机分为伪手术组、模型组、银杏内酯注射液组(3 mg/kg)、杏芎氯化钠注射液高(14 mg/kg)、低(7 mg/kg)剂量组，除伪手术组外其他大鼠均采用线栓法制备局灶性脑缺血再灌注损伤模型，尾静脉给药3 d，检测大鼠脑神经功能学损伤程度、脑组织含水量、脑梗死面积、脑组织病理形态学改变及脑组织生化学指标。结果表明，与模型组比较，杏芎氯化钠注射液在7和14 mg/kg的剂量下能明显改善脑缺血再灌注所致的大鼠神经功能损伤，降低脑含水量、减小脑梗死面积；此外14 mg/kg杏芎氯化钠注射液剂量组能显著改善脑病理组织学改变，降低脑组织中H₂O₂和丙二醛(MDA)含量，提高微量还原型谷胱甘肽(GSH)含量、谷胱甘肽过氧化物酶(GSH-Px)活力、超氧化物歧化酶(SOD)活力及抵抗超氧阴离子自由基和羟自由基的能力。杏芎氯化钠注射液可提高脑组织抗氧化能力，明显改善大鼠脑缺血再灌注损伤。
- 杏芎氯化钠注射液 /
- 川芎嗪 /
- 脑缺血再灌注 /
- 中动脉缺血再灌注模型 /
- 抗氧化
Abstract: The purpose of this research was to study the effect of Xingxiong sodium chloride injection on cerebral ischemia-reperfusion injury in rats. Rats were divided into five groups, namely sham group, model group, ginkgolide injection group(3 mg/kg), Xingxiong sodium chloride injection high-dose group(14 mg/kg)and low dose group(7 mg/kg). Except for the sham group, animals in other groups were subjected to ischemia for 2h and reperfusion for 72 h by middle cerebral artery occlusion(MCAO)with thread technique, and then drugs were administered continuously by tail intravenous injection during reperfusion period for 3 days. The neurological deficit score and the histopathological score were evaluated; infarction ratio, brain water content and biochemical indexes of animals in each group were determined. Compared with model group, reduction of neurological deficit score, brain water content and infarction area were observed obviously at 7 mg/kg and 14 mg/kg of Xingxiong sodium chloride injection. Additionally, reduction of histopathological score, H₂O₂ and MDA content, increase of the level of GSH, GSH-Px, SOD and also the capacity of inhibition of superoxide anion radical(·O^-₂)and hydroxyl radial(·OH)were observed at 14 mg/kg. The results suggested that Xingxiong sodium chloride injection could effectively enhance the ability of anti-oxidation in the brain tissues, and protect brain from ischemia-reperfusion injury.
- Xingxiong sodium chloride injection /
- ligustrazine /
- cerebral ischemia-reperfusion /
- middle cerebral artery occlusion reperfusion model /
- antioxidation

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参考文献(18)

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文章访问数: 1328
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杏芎氯化钠注射液对大鼠脑缺血再灌注损伤的保护作用及机制

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Protective effects and possible mechanism of Xingxiong sodium chloride injection on cerebral ischemia-reperfusion injury in rats

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