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参麦注射液对脓毒症小鼠炎症的抑制作用

柴营营, 曹丽娟, 张浩文, 陈寒昱, 王广基, 郝海平

柴营营, 曹丽娟, 张浩文, 陈寒昱, 王广基, 郝海平. 参麦注射液对脓毒症小鼠炎症的抑制作用[J]. 中国药科大学学报, 2016, 47(1): 79-83. DOI: 10.11665/j.issn.1000-5048.20160111
引用本文: 柴营营, 曹丽娟, 张浩文, 陈寒昱, 王广基, 郝海平. 参麦注射液对脓毒症小鼠炎症的抑制作用[J]. 中国药科大学学报, 2016, 47(1): 79-83. DOI: 10.11665/j.issn.1000-5048.20160111
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CHAI Yingying, CAO Lijuan, ZHANG Haowen, CHEN Hanyu, WANG Guangji, HAO Haiping. Shenmai injection inhibits inflammatory response in lipopolysaccharides-induced septic mice[J]. Journal of China Pharmaceutical University, 2016, 47(1): 79-83. DOI: 10.11665/j.issn.1000-5048.20160111
Citation: CHAI Yingying, CAO Lijuan, ZHANG Haowen, CHEN Hanyu, WANG Guangji, HAO Haiping. Shenmai injection inhibits inflammatory response in lipopolysaccharides-induced septic mice[J]. Journal of China Pharmaceutical University, 2016, 47(1): 79-83. DOI: 10.11665/j.issn.1000-5048.20160111
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参麦注射液对脓毒症小鼠炎症的抑制作用

  • 1.

    中国药科大学药代动力学重点实验室

  • 2.

    南京中医药大学

基金项目: 国家自然科学基金资助项目(No.81430091)

Shenmai injection inhibits inflammatory response in lipopolysaccharides-induced septic mice

摘要

    摘要
  • 摘要: 基于脓毒症全身炎症反应综合征的特点，探讨参麦注射液(Shenmai injection，SMI)对脂多糖(LPS)诱导的脓毒症小鼠的炎症干预作用并考察雌雄差异。分别将雌、雄小鼠按体重随机分为空白对照组、LPS诱导的脓毒症模型组和SMI干预组。干预组腹腔注射SMI 14 d，再给予10 mg/kg LPS诱导脓毒症，考察小鼠生存率、血清炎症因子、小鼠体内重要脏器中炎症因子的mRNA表达以及脏器组织干湿比评价SMI的干预效果，并对比雌雄差异。结果表明，注射SMI可以明显抑制LPS诱导的小鼠脓毒症，并有显著的雌雄差异，表现为对雌鼠保护作用更显著；SMI可以不同程度的抑制雄鼠和雌鼠体内重要脏器中主要促炎因子IL-6、IL-1β、TNF-α mRNA的表达，表现为IL-6 mRNA下调最显著，并与血清ELISA结果一致；通过考察组织干湿比发现，SMI对肝脏保护作用最显著。由此得出:SMI可通过不同程度调控LPS诱导的脓毒症小鼠体内主要脏器组织中炎症因子的表达，从而抑制血清中炎症因子分泌，进而显著降低其死亡率，且雌性小鼠保护作用更显著。
    Abstract: This study was to investigate the regulation of lipopolysaccharides(LPS)-induced sepsis in mice by preadministration of Shenmai injection(SMI)and the therapeutic differences between male and female, female and male mice were randomly grouped by weight, including control group, LPS-induced sepsis model group and SMI administration group. After preadministration of SMI for 14 days, 10 mg/kg LPS were intraperitoneally injected subsequently to induce sepsis. The survival rate of mice, level of serum cytokines and the mRNA expression of proinflammatory cytokines in main tissues were detected to evaluate the impact of SMI on LPS-induced sepsis mice. From the survival rate, which is considered as a gold standard of improvement in sepsis, significant protective effect can be observed after SMI pretreatment in LPS-induced sepsis mice, with a more significant effect shown in the females. Consisting with the serum cytokines levels, SMI significantly inhibited proinflammatory cytokines including IL-6, IL-1β and TNF-α mRNA expression in tissues and the regulation of IL-6 was most significant, which was consistent with the results of ELISA in serum. Moreover, the liver tissue acquired a more evident impact than any other tissues, which fits with the ratio of dry/wet weight. SMI can significantly inhibit inflammatory response by delivery in advance in LPS-induced septic mice, which provides strong evidence for elaborating the mechanism in the treatment of cardiovascular disease-related inflammation and shock.
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    • 参考文献(14)
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  • 刊出日期:  2016-02-24

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