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生脉散对人肠道菌群失衡的调节作用

花海莹, 李雪晴, 刘吉华

花海莹, 李雪晴, 刘吉华. 生脉散对人肠道菌群失衡的调节作用[J]. 中国药科大学学报, 2016, 47(1): 95-100. DOI: 10.11665/j.issn.1000-5048.20160114
引用本文: 花海莹, 李雪晴, 刘吉华. 生脉散对人肠道菌群失衡的调节作用[J]. 中国药科大学学报, 2016, 47(1): 95-100. DOI: 10.11665/j.issn.1000-5048.20160114
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HUA Haiying, LI Xueqing, LIU Jihua. In vitro study on the regulation of Shengmai San on dysbacteriosis of intestinal flora[J]. Journal of China Pharmaceutical University, 2016, 47(1): 95-100. DOI: 10.11665/j.issn.1000-5048.20160114
Citation: HUA Haiying, LI Xueqing, LIU Jihua. In vitro study on the regulation of Shengmai San on dysbacteriosis of intestinal flora[J]. Journal of China Pharmaceutical University, 2016, 47(1): 95-100. DOI: 10.11665/j.issn.1000-5048.20160114
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生脉散对人肠道菌群失衡的调节作用

  • 中国药科大学江苏省中药评价与转化重点实验室

基金项目: 国家自然科学基金资助项目(No.81173471)

In vitro study on the regulation of Shengmai San on dysbacteriosis of intestinal flora

摘要

    摘要
  • 摘要: 通过建立头孢曲松钠所致人肠道菌群紊乱体外模型，结合菌群的选择性培养、平板计数及短链脂肪酸分析，研究经典名方生脉散对肠道菌群失调的调节作用。研究结果显示，生脉散高中低剂量(16.5，11.2，5.5 mg/mL)组能显著抑制肠杆菌、肠球菌的增殖，促进肠道益生菌乳酸杆菌、梭菌增殖。与模型组相比，给予中剂量生脉散培养24 h后，肠球菌、肠杆菌增殖分别被抑制了24.9%和19.1%，乳酸杆菌和梭菌的增殖分别提高了22.3%和25.8%。同时生脉散各剂量组均能显著促进肠道菌群产生丙酸和乙酸，抑制潜在致病菌的增殖，促进失衡肠道菌群向正常菌群结构发展。
    Abstract: To study the regulation effect of Shengmai San(SMS)on intestinal dysbacteriosis, ceftriaxone sodium was used to induce intestinal dysbacteriosis in vitro, together with bacteria cultured in the selective medium and enumerated by plate counting, and the short chain fatty acid were also analyzed. The results showed that SMS(16. 5, 11. 2, 5. 5 mg/mL)could significantly inhibit the proliferation of such potentially harmful bacteria as Enterococci and Enterobacteriaceae, while promoting the proliferation of Lactobacillus and Clostridum. Compared with the model group, the proliferation of Enterococci and Enterobacteriaceae was inhibited by 24. 9% and 19. 1%, while the proliferation of Lactobacillus and Clostridum was increased by 22. 3% and 25. 8%, respectively, in the medium dose of SMS group(11. 2 mg/mL)at 24 h. Meanwhile, SMS in different concentrations significantly increased the accumulation of acetic acid and propionic acid generated by gut microbes which could significantly inhibit the proliferation of harmful bacteria. SMS showed probiotic effects on intestinal microflora imbalance induced by antibiotics in vitro.
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  • 刊出日期:  2016-02-24

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