高级检索

热驱动自组装白蛋白纳米粒的制备及体外评价

李芳, 姜力群, 辛俊勃, 郑春丽, 朱家壁, 刘建平

李芳, 姜力群, 辛俊勃, 郑春丽, 朱家壁, 刘建平. 热驱动自组装白蛋白纳米粒的制备及体外评价[J]. 中国药科大学学报, 2016, 47(3): 303-310. DOI: 10.11665/j.issn.1000-5048.20160310
引用本文: 李芳, 姜力群, 辛俊勃, 郑春丽, 朱家壁, 刘建平. 热驱动自组装白蛋白纳米粒的制备及体外评价[J]. 中国药科大学学报, 2016, 47(3): 303-310. DOI: 10.11665/j.issn.1000-5048.20160310
shu
LI Fang, JIANG Liqun, XIN Junbo, ZHENG Chunli, ZHU Jiabi, LIU Jianping. Preparation and in vitro evaluation of albumin nanoparticles produced by thermal driven self-assembly[J]. Journal of China Pharmaceutical University, 2016, 47(3): 303-310. DOI: 10.11665/j.issn.1000-5048.20160310
Citation: LI Fang, JIANG Liqun, XIN Junbo, ZHENG Chunli, ZHU Jiabi, LIU Jianping. Preparation and in vitro evaluation of albumin nanoparticles produced by thermal driven self-assembly[J]. Journal of China Pharmaceutical University, 2016, 47(3): 303-310. DOI: 10.11665/j.issn.1000-5048.20160310
shu

热驱动自组装白蛋白纳米粒的制备及体外评价

  • 1.

    中国药科大学药剂学教研室

  • 2.

    盐城卫生职业技术学院药学院

  • 3.

    徐州医科大学药学院

基金项目: 国家自然科学基金资助项目(No.81402878)；江苏省普通高校研究生科研创新计划项目(No.CXZZ13_0327)

Preparation and in vitro evaluation of albumin nanoparticles produced by thermal driven self-assembly

摘要

    摘要
  • 摘要: 探讨热驱动自组装白蛋白纳米粒的制备方法，并对其形成机制、细胞摄取、细胞摄取动力学与降解动力学等进行考察。通过测定巯基、氨基与羧基的浓度揭示白蛋白纳米粒的形成机制。采用CCK-8法检测纳米粒的细胞毒性，通过倒置荧光显微镜观察纳米粒的摄取行为，采用荧光共振能量转移(FRET)法测定白蛋白纳米粒在细胞内的摄取与降解动力学，并以紫杉醇为模型药物考察其对疏水性药物的包载能力。结果表明，热驱动自组装法制得的白蛋白纳米粒依靠分子间二硫键与酰胺键而稳定，安全无毒，易于被肿瘤细胞摄取并在胞内降解。载药实验结果显示，该纳米粒对紫杉醇有较高的包载。因此，热驱动自组装法绿色环保、操作简便，制得的白蛋白纳米粒可作为抗肿瘤药物传递的新平台。
    Abstract: The aim of this study was to prepare albumin nanoparticles by thermal driven self-assembly, and to investigate the formation mechanism, cellular uptake, the kinetics of cellular uptake and intracellular degradation, etc. By measuring the concentrations of thiol, amino and carboxyl groups, the formation mechanism of albumin nanoparticles was revealed. CCK-8 assay was performed to detect the cytotoxicity; inverted fluorescence microscope was used to observe the cellular uptake of the nanoparticles; while the fluorescence resonance energy transfer(FRET)method was applied to investigate the cellular uptake and intracellular degradation kinetics. The drug-loading capacity was investigated using paclitaxel(PTX)as the model drug. The results showed that the albumin nanoparticles produced by thermal driven self-assembly were safe, nontoxic, biodegradable and stabilized by intermolecular disulfide and amide bonds. The drug-loading study indicates that PTX can be highly encapsulated in the nanoparticles. Hence, thermal driven self-assembly method is green and easy to operate, and the albumin nanoparticles can be applied as a new delivery platform for anticancer drugs.
    • HTML全文
    • 参考文献(17)
  • [1] Carter DC,Ho JX.Structure of serum albumin[J].Adv Protein Chem,1994,45:153-203.
    [2] Elzoghby AO,Samy WM,Elgindy NA.Albumin-based nanoparticles as potential controlled release drug delivery systems[J].J Control Release, 2012,157(2):168-182.
    [3] Jenita JL,Chocalingam V,Wilson B.Albumin nanoparticles coated with polysorbate 80 as a novel drug carrier for the delivery of antiretroviral drug—Efavirenz[J].Int J Pharm Investig,2014,4(3):142-148.
    [4] Kim TH,Jiang HH,Youn YS,et al.Preparation and characterization of water-soluble albumin-bound curcumin nanoparticles with improved antitumor activity[J].Int J Pharm,2011,403(1/2):285-291.
    [5] Xu R,Fisher M,Juliano RL.Targeted albumin-based nanoparticles for delivery of amphipathic drugs[J].Bioconjug Chem,2011,22(5):870-878.
    [6] Gong J,Huo M,Zhou J,et al.Synthesis,characterization,drug-loading capacity and safety of novel octyl modified serum albumin micelles[J].Int J Pharm,2009,376(1/2):161-168.
    [7] Steinhauser IM,Langer K,Strebhardt KM,et al.Effect of trastuzumab-modified antisense oligonucleotide-loaded human serum albumin nanoparticles prepared by heat denaturation[J].Biomaterials,2008,29(29):4022-4028.
    [8] Weber C,Coester C,Kreuter J,et al.Desolvation process and surface characterisation of protein nanoparticles[J].Int J Pharm,2000,194(1):91-102.
    [9] Zhang L,Lu Z,Li X,et al.Methoxy poly(ethylene glycol)conjugated denatured bovine serum albumin micelles for effective delivery of camptothecin[J].Polym Chem,2012,3(8):1958-1961.
    [10] Deng W,Li J,Yao P,et al.Green preparation process,characterization and antitumor effects of doxorubicin-BSA-dextran nanoparticles[J].Macromol Biosci,2010,10(10):1224-1234.
    [11] Qi J,Yao P,He F,et al.Nanoparticles with dextran/chitosan shell and BSA/chitosan core—doxorubicin loading and delivery[J].Int J Pharm,2010,393(1/2):176-184.
    [12] Jiang L,Xu Y,Liu Q,et al.A nontoxic disulfide bond reducing method for lipophilic drug-loaded albumin nanoparticle preparation:formation dynamics,influencing factors and formation mechanisms investigation[J].Int J Pharm,2013,443(1/2):80-86.
    [13] Asghar S,Salmani JM,Hassan W,et al.A facile approach for crosslinker free nano self assembly of protein for anti-tumor drug delivery:factors′ optimization,characterization and in vitro evaluation[J].Eur J Pharm Sci,2014,63:53-62.
    [14] Erel O,Neselioglu S.A novel and automated assay for thiol/disulphide homeostasis[J].Clin Biochem,2014,47(18):326-332.
    [15] Zhou LQ,Liu JP,Xiong F,et al.Preparation and in vitro evaluation of PEG-coated superparamagnetic iron oxide nanoparticles[J].J China Pharm Univ(中国药科大学学报),2013,44(4):316-320.
    [16] Jiang L,Zhao X,Zheng C,et al.The quantitative detection of the uptake and intracellular fate of albumin nanoparticles[J].RSC Advances,2015,44(5):34956-34966.
    [17] deWit JN,Klarenbeek G.Effects of various heat treatments on structure and solubility of whey proteins[J].J Dairy Sci,1984,67(11):2701-2710.
    • 相关文章
    • 施引文献
    • 资源附件(0)
计量
  • 文章访问数: 
  • HTML全文浏览量:  0
  • PDF下载量: 
  • 被引次数: 0
出版历程
  • 刊出日期:  2016-06-24

目录

摘要
HTML全文
    参考文献(17)
    相关文章
    施引文献
    资源附件(0)

    /

    返回文章
    返回

    版权所有：《中国药科大学学报》编辑部苏ICP备05007142号

    地址：江苏省南京市鼓楼区童家巷24号（210009）电话: 025-83271566E-mail: xuebao@cpu.edu.cn

    本系统由 北京仁和汇智信息技术有限公司 开发  百度统计

    访问量：326312

    x 关闭 永久关闭