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远志-石菖蒲药对配伍前后远志化学成分定性定量分析

吕广云, 王双双, 杭太俊, 宋敏

吕广云, 王双双, 杭太俊, 宋敏. 远志-石菖蒲药对配伍前后远志化学成分定性定量分析[J]. 中国药科大学学报, 2016, 47(3): 329-336. DOI: 10.11665/j.issn.1000-5048.20160314
引用本文: 吕广云, 王双双, 杭太俊, 宋敏. 远志-石菖蒲药对配伍前后远志化学成分定性定量分析[J]. 中国药科大学学报, 2016, 47(3): 329-336. DOI: 10.11665/j.issn.1000-5048.20160314
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LYU Guangyun, WANG Shuangshuang, HANG Taijun, SONG Min. Qualitative and quantitative analysis of components of Radix Polygalae in herbal pair of Radix Polygalae and Rhizoma Acori Tatarinowii[J]. Journal of China Pharmaceutical University, 2016, 47(3): 329-336. DOI: 10.11665/j.issn.1000-5048.20160314
Citation: LYU Guangyun, WANG Shuangshuang, HANG Taijun, SONG Min. Qualitative and quantitative analysis of components of Radix Polygalae in herbal pair of Radix Polygalae and Rhizoma Acori Tatarinowii[J]. Journal of China Pharmaceutical University, 2016, 47(3): 329-336. DOI: 10.11665/j.issn.1000-5048.20160314
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远志-石菖蒲药对配伍前后远志化学成分定性定量分析

  • 1.

    中国药科大学药物分析系

  • 2.

    中国药科大学药物质量与安全预警教育部重点实验室

Qualitative and quantitative analysis of components of Radix Polygalae in herbal pair of Radix Polygalae and Rhizoma Acori Tatarinowii

摘要

    摘要
  • 摘要: 研究药对远志-石菖蒲配伍对远志药材化学成分的影响。采用HPLC-Q-TOF-MS/MS法对远志-石菖蒲配伍前后远志化学成分进行定性，并用HPLC-UV法进行定量分析。采用C18色谱柱(250 mm×4.6 mm，5 μm)，以乙腈-0.1%甲酸水溶液为流动相梯度洗脱；质谱使用电喷雾离子源，在正离子模式下扫描；通过对照品对照、质谱数据和对比文献对远志化学成分的各色谱峰进行归属，并比较远志-石菖蒲配伍前后远志各化学成分的含量变化。从远志中鉴定出8个化学成分:3，4，5-三甲氧基肉桂酸、对甲氧基肉桂酸、细叶远志皂苷、西伯利亚远志糖A5、远志GFEA9酮Ⅲ、细叶远志苷B、3，6′-二芥子酰基蔗糖和细叶远志苷A；经石菖蒲配伍后，远志的8个化学成分含量无明显变化。远志-石菖蒲配伍后的化学成分定性定量研究可为该药对的体内配伍作用探索提供方法依据。
    Abstract: The purpose of this research was to explore the differences of the components of Radix Polygalae in herbal pair of Radix Polygalae and Rhizoma Acori Tatarinowii. An HPLC-Q-TOF-MS/MS method and an HPLC-UV method were established for the identification and determination of the components of Radix Polygalae, respectively. HPLC separation was carried out on a C18 column(250 mm×4. 6 mm, 5 μm)with linear gradient elution using a mobile phase consisting of acetonitrile and 0. 1% formic acid aqueous solution. Mass spectrometry with ESI source was performed in the positive ion mode to scan MS data including total ion chromatograms and ion peaks of Radix Polygalae. Eight components including 3, 4, 5-trimethoxycinnamic acid, p-methoxycinnamic acid, tenuifolin, sibiricose A5, polygalaxanthone III, tenuifoliside B, 3, 6′-disinapoly sucrose, and tenuifoliside A were identified according to the reference substance retention time, MS data and literatures. There was no significant variation found in the contents of eight chemical constituents of Radix Polygalae. The qualification and quantitation study of the components in herbal pair of Radix Polygalae and Rhizoma Acori Tatarinowii provide the methodological basis for compatibility mechanism exploration in vivo.
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    • 参考文献(14)
  • [1] Lyu JS.Study of SHI Jin-mo′s Pair Drugs(施今墨对药)[M].3rd ed.Beijing:People′s Military Medical Press,2007:265-266.
    [2] Lyu HM,Zhu C,Liao YJ,et al.Tenuigenin ameliorates acute lung injury by inhibiting NF-κB and MAPK signalling pathways[J].Resp Physiol Neurobi,2015,216:43-51.
    [3] Tian F, Jiang Y, Zhang K, et al. The effect and mechanism of Polygala tenuifolia glycoside on SAM-P/8[J].Geriatr Health Care(老年医学与保健),2004,10(3):137-139.
    [4] Chen Q,Cao YG,Zhang CH.Effect of tenuigenin on cholinergic decline induced by β-amyloid peptide and ibotenic acid in rats[J].Acta Pharm Sin(药学学报),2002,37(12):913-917.
    [5] Nikaido T,Ohmoto T,Saitoh H,et al.Inhibitors of cyclic adenosine monophosphate phosphodiesterase in Polygala tenuifolia[J].Chem Pharm Bull,1982,30(30):2020-2024.
    [6] Chinese Pharmacopoeia Commission.Chinese Pharmacopoeia:part 1(中华人民共和国药典:一部)[S].Beijing:China Medical Science Press,2015:156-157.
    [7] Li J,Dong XB,Jiang Y,et al.HPLC determination of total saponins in Radix Polygalae[J].Chin J Pharm Anal(药物分析杂志),2007,27(9):1329-1332.
    [8] Yang XD,Xu LZ,Yang SL.Advances in the research of chemistry and pharmacology of xanthones extracted from Polygala L.[J].Nat Prod Res Dev(天然产物研究与开发),2000,12(5):88-94.
    [9] Liu P,Wang DX,Guo DH,et al.Antidepressant effect of 3,6′-disinapoyl sucrose from Polygala tenuifolia Willd.in pharmacological depression model[J].Chin Pharm J(中国药学杂志),2008,43(18):1391-1394.
    [10] Tu HH,Liu P,Mu L,et al.Study on antidepressant components of sucrose ester from Polygala tenuifolia[J].Chin J Chin Mater Med(中国中药杂志),2008,33(11):1278-1280.
    [11] Zhang WJ,Zheng XH,Fang MF,et al.Analysis of diversify in matched pair of Polygala tenuifolia Willd.and Acorus Tatarinowii Schott by HPLC[J].China Pharm(中国药业),2009,18(15):6-7.
    [12] Wang J, Liu XM, Chang Q, et al. Chemical constituents from alkaline hydrolysis products of total saponins in Radix Palygalae[J].Cent South Pharm(中南药学),2010,8(11):811-814.
    [13] Ikeya Y,Sugama K,Okada M,et al.Four new phenolic glycosides from Polygala tenuifolia[J].Chem Pharm Bull,1991,39(10):2600-2605.
    [14] Jiang Y,Tu PF.Studies on the chemical constituents in root bark of Polygala tenuifolia(Ⅱ)[J].China J Chin Mater Med(中国中药杂志),2004,29(8):751-753.
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  • 刊出日期:  2016-06-24

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