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姜黄素对高糖诱导的L6细胞线粒体功能障碍的改善作用

薛春玲, 诸葛远莉, 曾静霞

薛春玲, 诸葛远莉, 曾静霞. 姜黄素对高糖诱导的L6细胞线粒体功能障碍的改善作用[J]. 中国药科大学学报, 2016, 47(3): 342-347. DOI: 10.11665/j.issn.1000-5048.20160316
引用本文: 薛春玲, 诸葛远莉, 曾静霞. 姜黄素对高糖诱导的L6细胞线粒体功能障碍的改善作用[J]. 中国药科大学学报, 2016, 47(3): 342-347. DOI: 10.11665/j.issn.1000-5048.20160316
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XUE Chunling, ZHUGE Yuanli, ZENG Jingxia. Improved effect of curcumin on mitochondrial dysfunction induced by high glucose in L6 cells[J]. Journal of China Pharmaceutical University, 2016, 47(3): 342-347. DOI: 10.11665/j.issn.1000-5048.20160316
Citation: XUE Chunling, ZHUGE Yuanli, ZENG Jingxia. Improved effect of curcumin on mitochondrial dysfunction induced by high glucose in L6 cells[J]. Journal of China Pharmaceutical University, 2016, 47(3): 342-347. DOI: 10.11665/j.issn.1000-5048.20160316
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姜黄素对高糖诱导的L6细胞线粒体功能障碍的改善作用

  • 1.

    南京医科大学附属南京儿童医院

  • 2.

    南京中医药大学附属南京市中西医结合医院

Improved effect of curcumin on mitochondrial dysfunction induced by high glucose in L6 cells

摘要

    摘要
  • 摘要: 考察姜黄素对高糖诱导的L6细胞线粒体功能障碍的改善作用。采用40 mmol/L葡萄糖处理24 h，建立L6细胞线粒体功能障碍模型，姜黄素受试浓度分别为10、20、40 μmol/L。结果显示，高糖培养可引起L6细胞线粒体功能出现明显障碍，主要表现为明显的线粒体膜电位降低、活性氧增多、ATP含量减少，线粒体生物合成(mtDNA数量)部分下降，同时UCP2 、PGC-1α和Sirt3 mRNA和蛋白表达下调。姜黄素对于高糖所致线粒体功能障碍(膜电位下降，活性氧增多、ATP含量减少)具有明显的改善作用，同时升高UCP2的mRNA和蛋白表达，对于线粒体生物合成(mtDNA数量)未见明显影响。本研究提示姜黄素可能是通过多种途径的抗氧化作用而发挥线粒体保护作用。
    Abstract: To investigate the effects of curcumin on mitochondrial dysfunction induced by high glucose(40 mmol/L glucose, 24 h)in L6 cells, curcumin(10, 20, 40 μmol/L)was administered for 24 h after high glucose culture. The effects of curcumin on the mitochondrial dysfunction were evaluated by mitochondrial membrane potential, reactive oxygen species(ROS), ATP content and mtDNA copy number. The mRNA and protein expression of uncoupling protein 2(UCP2), PPARγ coactivator 1α(PGC-1α)and sirtuin-1(Sirt3)were also determined. As improvement of high glucose damage, curcumin significantly raised mitochondrial membrane potential and ATP content, and decreased ROS level. Curcumin significantly ameliorated the down regulation of UCP2 yet with little effect on mtDNA copy number and PGC-1α and Sirt3 expression. In conclusion, curcumin could significantly ameliorate mitochondrial dysfunction in L6 cells induced by high glucose, which involved the mechanism of multiple antioxidants.
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    • 参考文献(16)
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  • 刊出日期:  2016-06-24

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