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金芪降糖片对糖尿病心血管并发症的治疗作用

赵有红, 杨洋, 谢治深, 徐晓军, 刘莉

赵有红, 杨洋, 谢治深, 徐晓军, 刘莉. 金芪降糖片对糖尿病心血管并发症的治疗作用[J]. 中国药科大学学报, 2016, 47(3): 348-352. DOI: 10.11665/j.issn.1000-5048.20160317
引用本文: 赵有红, 杨洋, 谢治深, 徐晓军, 刘莉. 金芪降糖片对糖尿病心血管并发症的治疗作用[J]. 中国药科大学学报, 2016, 47(3): 348-352. DOI: 10.11665/j.issn.1000-5048.20160317
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ZHAO Youhong, YANG Yang, XIE Zhishen, XU Xiaojun, LIU Li. Protective effects of Jinqi Jiangtang tablets on diabetic complications of cardiovascular diseases in Drosophila[J]. Journal of China Pharmaceutical University, 2016, 47(3): 348-352. DOI: 10.11665/j.issn.1000-5048.20160317
Citation: ZHAO Youhong, YANG Yang, XIE Zhishen, XU Xiaojun, LIU Li. Protective effects of Jinqi Jiangtang tablets on diabetic complications of cardiovascular diseases in Drosophila[J]. Journal of China Pharmaceutical University, 2016, 47(3): 348-352. DOI: 10.11665/j.issn.1000-5048.20160317
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金芪降糖片对糖尿病心血管并发症的治疗作用

  • 1.

    南京医科大学第一附属医院

  • 2.

    中国药科大学天然药物国家重点实验室

Protective effects of Jinqi Jiangtang tablets on diabetic complications of cardiovascular diseases in Drosophila

摘要

    摘要
  • 摘要: 探讨金芪降糖片对糖尿病心血管并发症的治疗作用。将健康果蝇随机分为3组，分别给予正常饮食、10%高脂饮食、1%金芪降糖片+10%高脂饮食，2周后用试剂盒测量果蝇海藻糖、三酰甘油的浓度，高速电子倍增摄像机拍摄果蝇心管搏动视频测量心功能，定量PCR测量果蝇心管4ebp、pepck RNA表达量，Western blot测定磷酸化4EBP蛋白表达量。结果显示，高脂饮食可引起果蝇海藻糖、三酰甘油升高，并导致心功能损伤，而金芪降糖片治疗后可以明显降低糖脂水平及恢复心功能；同时与高脂组相比，受试药可提高心血管4ebpRNA表达量及降低pepck RNA表达量，降低果蝇磷酸化4EBP蛋白表达量。结果表明，受试药具有较好的降糖降脂及恢复心功能作用，且可能通过mTOR/4EBP信号通路发挥作用。
    Abstract: This study was to investigate the protective effects of Jinqi Jiangtang tablets on diabetic complications of cardiovascular disease in Drosophila. The effects of Jinqi Jiangtang tablets on hypolipidemia and hypoglycemia by high fat diet(HFD)induced model organism Drosophila with the content of triglyceride and trehalose in Drosophila as indexes, were investigated. And the Drosophila heart function was detected by high speed EM-CCD; cell signaling pathways were detected by Western blot and RT-PCR. The results showed that high fat diet could induce the appearance of hyperglycemia and hyperlipidemia on Drosophila model. Jinqi Jiangtang tablets could significantly reduce triglyceride and trehalose and protect heart function of Drosophila induced by high fat diet. Jinqi Jiangtang tablets could also increase the expression of 4ebp mRNA, while decreasing p-4EBP protein and pepck mRNA expression. This study demonstrated that Jinqi Jiangtang tablets have a protective effect on HFD-induced dyslipidemic-diabetic and cardiac dysfunction, which may be related to mTOR/4EBP pathway.
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    • 参考文献(12)
  • [1] Peng JL,Yin P,Wang B, et al.Efficacy and safety of Jinqi Jiangtang tablet in treatment of type 2 diabetes mellitus[J].Her Med(医药导报),2013,32(6):796-800.
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    [3] Xu X,Gopalacharyulu P,Seppanen-Laakso T, et al.Insulin signaling regulates fatty acid catabolism at the level of CoA activation[J].PLoS Genetics,2012,8(1):e1002478.
    [4] Ocorr K,Reeves NL,Wessells RJ, et al.KCNQ potassium channel mutations cause cardiac arrhythmias in Drosophila that mimic the effects of aging[J].Proc Natl Acad Sci U S A,2007,104(10):3943-3948.
    [5] Zhu J,Jin X,Rei J, et al.Protection effect of new technological Jin Qi Jiang Tang tablets on rat pancreatic islet cells[J].Acta Acad Med CPAF(武警医学院学报),2010,19(6):437-441.
    [6] Yang FY,Yu J,Luo G, et al.The effect of new technological Jin Qi Jiang Tang tablets on rat glucose and lipid metabolism[J].Lishizhen Med Mater Med Res(时珍国医国药),2012,23(3):677-678.
    [7] Wang R,Wu J.Research progress on the effect of Jinqi Jiangtang tablet on protecting vascular endothelium diabetic nephropathy and insulin resistance[J].World Chin Med(世界中医药),2014,9(2):261-263.
    [8] Zheng N,Wei SJ.Protective effect of Jin Qi Jiang Tang tablets on kidney in diabetic rats[J].Tianjin J Tradit Chin Med(天津中医药),2014,31(4):226-230.
    [9] Laplante M,Sabatini DM.mTOR signaling at a glance[J].J Cell Sci, 2009,122(20):3589-3594.
    [10] Harris TE,Lawrence JC Jr.TOR signaling[J].Sci STKE,2003,2003(212):re 15.
    [11] Zhang HM, Fu J, Hamilton R, et al. The mammalian target of rapamycin modulates the immunoproteasome system in the heart[J].J Mol Cell Cardiol, 2015,86:158-167.
    [12] Johnson SC,Rabinovitch PS,Kaeberlein M.mTOR is a key modulator of ageing and age-related disease[J].Nature,2013,493(7432):338-345.
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  • 刊出日期:  2016-06-24

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