响应面法优化白背三七多糖的提取工艺

朱亚松; 许伟; 邵荣; 朱跃钊; 程德林; 廖传华

doi:10.11665/j.issn.1000-5048.20160319

响应面法优化白背三七多糖的提取工艺

朱亚松^1,2,3,4,
许伟^2,3,
邵荣^2,3,
朱跃钊¹,
程德林⁴,
廖传华¹

1.
南京工业大学机械与动力工程学院
2.
盐城工学院化学化工学院
3.
江苏省海洋滩涂生物化学与生物技术重点建设实验室
4.
盐城康林达生物科技有限公司

基金项目: 江苏省产学研前瞻性联合研究项目资助项目(No.BY2015057-29)；星火计划资助项目(No.2015GA690049)

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出版历程
- 刊出日期: 2016-06-24

Optimization of microwave extraction condition of Gynura divaricata polysaccharide by response surface analysis

摘要

摘要: 以白背三七为原料，采用微波辅助法提取白背三七多糖。在单因素实验的基础上，采用响应面法优化白背三七多糖的提取工艺。实验结果表明，影响微波辅助提取白背三七多糖的主要因素依次为:液料比、微波时间、微波功率，其中料液比对提取率的影响达到了极其显著水平。白背三七多糖提取的最佳工艺参数为:液料比45∶1(mL/g)、微波功率390 W、微波时间22 min。在此条件下，白背三七多糖的提取率为15.36%，与模型预测值接近。因此，采用响应曲面法优化得到的工艺条件具有一定的实际应用价值。
- 白背三七 /
- 多糖 /
- 微波 /
- 响应面
Abstract: Gynura divaricata polysaccharide was extracted from Gynura divaricata with the method of microwave-assisted extraction. Based on the results of single factor experiments, the extraction process was optimized by response surface analysis. The results indicated that the influencing factors during extraction were: liquid-solid ratio, microwave time, and microwave power, among which the influence of liquid-solid ratio was the most significant. The optimal conditions were as follows: liquid-solid ratio of 45∶1(mL/g), microwave power of 390 W, microwave time of 22 min. Under optimal extraction conditions, the extraction rate of Gynura divaricata polysaccharide was 15. 36%, which was pretty close to the predicted result. The optimized response surface methodology had some practical value for the process.
- Gynura divaricata /
- polysaccharide /
- microwave /
- response surface

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参考文献(10)

[1]	Hu XM,Zhang WK,Zhu QS.Chinese Materia Medica(中华本草)[M].Shanghai:Shanghai Science and Technology Publishing House,1999:43-44.
[2]	Liu L,Jia J,Zeng G,et al.Studies on immunoregulatory and anti-tumor activities of a polysaccharide from Salvia miltiorrhiza Bunge[J].Carbohydr Polym,2013,92(1):479-483.
[3]	Jiang MH,Hu JZ,Qiu WG,et al.Hypoglycemic and anti-anoxia effect of polysaccharide and flavonoids in Gynura divaricata(L.)DC.[J].Chin J Hosp Pharm(中国医院药学杂志),2009,13(29):1074-1076.
[4]	Liu WW,Liu X,Cao XL.Separation and purification of polysaccharides from leaves of Gynura davaricata(L.)DC.[J].Food Sci(食品科学),2012,32(23):58-63.
[5]	Zhao CH,Liu JF,Feng MY,et al.Effect of different extraction methods on the properties of longan polysaccharides[J].Mod Food Sci Tech(现代食品科技),2012,10(28):1298-1305.
[6]	Yu KC,Yang XJ,Li N,et al.Optimization of the extraction process of polysaccharide from Platycodon grandiflorum[J].J Sci Teach Coll Univ(高师理科学刊),2014,3(34):71-73.
[7]	Chen HS,Gao JS,Po S,et al.Study on extraction process of the head polysaccharides by homogeneous design optimization[J].Food Res Dev(食品研究与开发),2015,4(36):65-68.
[8]	Chen YT,Li YX,Huang L,et al.Extraction of polysacharides in potentilla discolor by microwave extraction[J].Anal Test Tech Inst(分析测试技术与仪器),2014,4(20):191-196.
[9]	Thirugnanasambandham K,Sivakumar V,Maran JP.Microwave-assisted extraction of polysaccharides from mulberry leaves[J].Int J Biol Macromol,2015,72:1-5.
[10]	Cheng ZY, Yang YJ, Liu ZG. Resarch on microwave-assisted extraction of polysacharide from Schisandra chinensis(Turcz.)Baill[J].J Jilin Inst Chem Tech(吉林化工学院学报),2014,31(3):18-22.