响应面法优化白背三七多糖的提取工艺

Optimization of microwave extraction condition of Gynura divaricata polysaccharide by response surface analysis

摘要: 以白背三七为原料，采用微波辅助法提取白背三七多糖。在单因素实验的基础上，采用响应面法优化白背三七多糖的提取工艺。实验结果表明，影响微波辅助提取白背三七多糖的主要因素依次为:液料比、微波时间、微波功率，其中料液比对提取率的影响达到了极其显著水平。白背三七多糖提取的最佳工艺参数为:液料比45∶1(mL/g)、微波功率390 W、微波时间22 min。在此条件下，白背三七多糖的提取率为15.36%，与模型预测值接近。因此，采用响应曲面法优化得到的工艺条件具有一定的实际应用价值。

Abstract: Gynura divaricata polysaccharide was extracted from Gynura divaricata with the method of microwave-assisted extraction. Based on the results of single factor experiments, the extraction process was optimized by response surface analysis. The results indicated that the influencing factors during extraction were: liquid-solid ratio, microwave time, and microwave power, among which the influence of liquid-solid ratio was the most significant. The optimal conditions were as follows: liquid-solid ratio of 45∶1(mL/g), microwave power of 390 W, microwave time of 22 min. Under optimal extraction conditions, the extraction rate of Gynura divaricata polysaccharide was 15. 36%, which was pretty close to the predicted result. The optimized response surface methodology had some practical value for the process.