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一种新型马鹿茸多肽纯化、鉴定及体外降血糖活性

姜宁, 张双健, 朱静, 尚靖, 高向东

姜宁, 张双健, 朱静, 尚靖, 高向东. 一种新型马鹿茸多肽纯化、鉴定及体外降血糖活性[J]. 中国药科大学学报, 2016, 47(3): 363-367. DOI: 10.11665/j.issn.1000-5048.20160320
引用本文: 姜宁, 张双健, 朱静, 尚靖, 高向东. 一种新型马鹿茸多肽纯化、鉴定及体外降血糖活性[J]. 中国药科大学学报, 2016, 47(3): 363-367. DOI: 10.11665/j.issn.1000-5048.20160320
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JIANG Ning, ZHANG Shuangjian, ZHU Jing, SHANG Jing, GAO Xiangdong. Purification, identification and hypoglycemic activity in vitro of a novel peptide from red deer antler[J]. Journal of China Pharmaceutical University, 2016, 47(3): 363-367. DOI: 10.11665/j.issn.1000-5048.20160320
Citation: JIANG Ning, ZHANG Shuangjian, ZHU Jing, SHANG Jing, GAO Xiangdong. Purification, identification and hypoglycemic activity in vitro of a novel peptide from red deer antler[J]. Journal of China Pharmaceutical University, 2016, 47(3): 363-367. DOI: 10.11665/j.issn.1000-5048.20160320
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一种新型马鹿茸多肽纯化、鉴定及体外降血糖活性

  • 1.

    中国药科大学生命科学与技术学院

  • 2.

    湖北民族学院生物科学与技术学院

  • 3.

    中国药科大学新药筛选中心

基金项目: 新疆维吾尔族自治区科技支疆资助项目(No.2008ZJ14)；科技人员服务企业行动资助项目(No.2009GJG40009)

Purification, identification and hypoglycemic activity in vitro of a novel peptide from red deer antler

摘要

    摘要
  • 摘要: 从马鹿茸干品中分离具有降血糖活性的多肽。以新疆塔里木马鹿茸干品为原料，粉碎后经醋酸-醋酸钠(pH 3.5)缓冲液浸提，再经醇沉、超滤、离子交换色谱、凝胶过滤色谱和反相色谱纯化多肽，并对该多肽进行MALDI TOF/MS相对分子质量检测和LC-MS/MS鉴定。MTT法检测促胰岛β细胞增殖和STZ损伤后修复能力以及建立肝细胞胰岛素耐受模型检测该多肽促肝细胞葡萄糖消耗量活性。结果表明，分离纯化得到的多肽CAP经MALDI-TOF/MS检测，其相对分子质量为6 804，LC-MS/MS及相关数据库比对发现，CAP为一种新的肽。CAP能显著促胰岛β细胞的增殖和STZ损伤后的修复，并能显著增加肝细胞的葡萄糖摄入量。
    Abstract: This study was aimed at isolating a peptide with hypoglycemic activity from dried red deer antler. A peptide from dried red deer antler of Tarim in Xingjiang was extracted with acetate buffer(pH 3. 5), followed by precipitation with ethanol, ultrafiltration and separation via ion exchange chromatography, gel filtration and reversed phase chromatography. The molecular mass of the peptide was detected via MALDI TOF/MS and the peptide was identified by LC-MS/MS. The activities of the peptide to promote islet β cells proliferation and recovery the damage induced by streptozotocin(STZ)were detected by MTT, and the effect of the peptide on glucose consumption of hepatocyte was determined. The peptide, named as CAP, was obtained from dried red deer antler through purification with the molecular mass of 6 804 via MALDI TOF/MS. The result of LC-MS/MS and the comparison in relative database showed that CAP was a novel peptide. CAP stimulated the proliferation of pancreatic cells and recovery of STZ-damaged pancreatic cells, as well as increased glucose consumption of HepG2.
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    • 参考文献(11)
  • [1] Chinese Pharmacopoeia Commission. Chinese Pharmacopoeia: part 1(中华人民共和国药典:一部)[S].Beijing:China Medical Science Press,2010:303.
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    [8] Shen T,Lee J,Lee E,et al.Cafestol,a coffee-specific diterpene,is a novel extracellular signal-regulated kinase inhibitor with AP-1-targeted inhibition of prostaglandin E2 production in lipopolysaccharide-activated macrophages[J].Biol Pharm Bull,2011,33(1):128-132.
    [9] Kim KC,Kim JS,Kang KA,et al.Cytoprotective effects of catechin 7-O-β-D glucopyranoside against mitochondrial dysfunction damaged by streptozotocin in RINm5F cells[J].Cell Biochem Funct,2010,28(8):651-660.
    [10] Iyer VV,Yang H,Ierapetritou MG,et al.Effects of glucose and insulin on HepG2-C3A cell metabolism[J].Biotechnol Bioeng,2010,107(2):347-356.
    [11] Zhou R,Wang F,Hao J,et al.Ultrafiltration and concentration used for separating insulin-like growth factor-1 from Cervi Cornu Pantotrichum[J].China Tradit Herb Drugs(中草药),2013,44(10):1257-1262.
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  • 刊出日期:  2016-06-24

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